基于GC-MS建立载气吹扫实验装置匹配测定电子烟雾尼古丁浓度

doi:10.12092/j.issn.1009-2501.2018.07.009

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (7): 776-781.doi: 10.12092/j.issn.1009-2501.2018.07.009

基于GC-MS建立载气吹扫实验装置匹配测定电子烟雾尼古丁浓度

张永学¹，何庆²，张晓宇²，张文欣¹，赵庆中¹，汪志伟¹，高杉¹

¹安徽医科大学基础医学院药理学教研室，合肥 230032，安徽;²安徽中烟工业有限责任公司技术中心，合肥 230088，安徽

收稿日期:2017-11-02 修回日期:2018-04-04 出版日期:2018-07-26 发布日期:2018-07-20
通讯作者: 高杉，男，教授，博士生导师，研究方向：心血管药理学。Tel:13083495662 E-mail:gaoshan@ahmu.edu.cn
作者简介:张永学，男，硕士，研究方向：心血管药理学。 Tel：15556993791 E-mail:yongxuezhang168@126.com
基金资助:
烟草化学安徽省重点实验室2014开放课题基金（2015034）

GC-MS-based a carrier gas purge experimental device to test the electronic smoke nicotine concentration

ZHANG Yongxue¹, HE Qing², ZHANG Xiaoyu², ZHANG Wenxin¹, ZHAO Qingzhong¹, WANG Zhiwei¹, GAO Shan¹

¹Department of Pharmacology, Anhui Medical University, Hefei 230032, Anhui, China；² Technical Center of Anhui Tobacco Industry Co., Ltd., Hefei 230088, Anhui, China

Received:2017-11-02 Revised:2018-04-04 Online:2018-07-26 Published:2018-07-20

摘要/Abstract

摘要：

目的: 建立匹配电子烟烟雾尼古丁浓度的实验装置。方法: 采用GC-MS检测尼古丁，建立尼古丁浓度与峰面积线性方程。同法测定不同流速空气为载气吹扫液态尼古丁形成气溶胶中尼古丁浓度。ELISA法测定尼古丁大鼠体内代谢物可替宁浓度。结果: 在0.226 0~2.269 7 μg/mL范围内，尼古丁峰面积与其质量浓度呈现良好线性关系，决定系数（r²=0.993 7）。在标准气态尼古丁浓度分别为0.567 4、1.134 8、1.702 3 μg/mL水平下，平均回收率分别为97.2%、113.7%、108.1%，相对标准差（RSD）分别为6.1%，20.8%，16.9%（n=3）。标准品与电子烟雾尼古丁保留时间均为13.41 min，均出现碎片定量离子m/z 84.03，辅助定量离子为m/z 133.03，方法专属性好。电子烟烟雾尼古丁浓度为0.584 μg/mL。条件: 25 ℃尼古丁液面高度为20 cm，空气流1.1 L/min时，实验装置内的气溶胶尼古丁浓度与电子烟烟雾匹配。与对照组血清中可替宁浓度(2.05±0.83) ng/mL相比，尼古丁组血清中可替宁浓度（5.31±0.62）ng/mL明显升高(P<0.01)。结论: 建立了与电子烟烟雾尼古丁浓度相匹配的实验装置，实验装置气体可以使大鼠感染尼古丁。

关键词: 载气吹扫法, 电子烟, 气相色谱质谱（GC-MS), 尼古丁, 可替宁

Abstract:

AIM: To establish an experimental device to test the nicotine concentration of cigarette smoke. METHODS: The nicotine concentration and peak area linear equation were established by GC-MS. It was used to determine the different flow rates of air for the carrier gas purge of liquid nicotine formation of aerosol nicotine concentration. Serum cotinine concentration in rats was determined by ELISA. RESULTS: In the range of 0.226 0 to 2.269 7 μg/mL, the nicotine peak area showed a good linear relationship with its mass concentration, and the correlation coefficient (r²=0.993 7).The average recoveries were 97.2%, 113.7%, and 108.1%, respectively, with standard gaseous nicotine concentrations of 0.567 4, 1.134 8, and 1.702 3 μg/mL, respectively. The relative standard deviations (RSD) were 6.1% and 20.8%,16.9%,respectively (n=3).The retention time of standard and e-cigarette nicotine was 13.41 min. Fragment quantification ion m/z 84.03 and auxiliary quantification ion m/z 133.03 appeared. The specificity of the method was good. Electronic cigarette smoke nicotine concentration was 0.584 μg/mL. Conditions: 25 ℃ nicotine liquid height of 20 cm, air flow 1.1 L/min, the experimental device aerosol nicotine concentration and electronic cigarette smoke matched. Compared with the control group (2.05±0.83) ng/mL, the serum cotinine concentration in the nicotine group was significantly higher(5.31±0.62) ng/mL (P<0.01). CONCLUSION: An experimental device is established to test the nicotine concentration of the electronic cigarette smoke,which can infect rats with nicotine.

Key words: gas purge method, e-cigarette, gas chromatography mass spectrometry (GC-MS), nicotine, cotinine

中图分类号:

R331

张永学，何庆，张晓宇，张文欣，赵庆中，汪志伟，高杉. 基于GC-MS建立载气吹扫实验装置匹配测定电子烟雾尼古丁浓度[J]. 中国临床药理学与治疗学, 2018, 23(7): 776-781.

ZHANG Yongxue, HE Qing, ZHANG Xiaoyu, ZHANG Wenxin, ZHAO Qingzhong, WANG Zhiwei, GAO Shan. GC-MS-based a carrier gas purge experimental device to test the electronic smoke nicotine concentration[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 776-781.

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基于GC-MS建立载气吹扫实验装置匹配测定电子烟雾尼古丁浓度

GC-MS-based a carrier gas purge experimental device to test the electronic smoke nicotine concentration

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