黄芪甲苷通过SDF-1/CXCR4信号通路诱导人肾腺癌细胞的凋亡

doi:10.12092/j.issn.1009-2501.2019.06.010

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (6): 665-669.doi: 10.12092/j.issn.1009-2501.2019.06.010

黄芪甲苷通过SDF-1/CXCR4信号通路诱导人肾腺癌细胞的凋亡

邹照银1，郑府¹，徐先顺¹，丁文娟¹，陈艳¹，蒲道静²

¹恩施土家族苗族自治州中心医院泌尿外科，²医院感染管理科，恩施 445000，湖北

收稿日期:2019-01-09 修回日期:2019-02-12 出版日期:2019-06-26 发布日期:2019-06-25
通讯作者: 蒲道静，女，本科，副主任护师，主要研究肾脏肿瘤防治工作。 E-mail：375642152@qq.com
作者简介:邹照银，女，本科，主要研究肾脏肿瘤防治工作。 Tel:13403009098 E-mail：819534548@qq.com

Effect of astragaloside on the apoptosis of human renal carcinoma cell (ACHN) via SDF-1/CXCR4 signaling pathway

ZOU Zhaoyin¹, ZHENG Fu¹, XU Xianshun ¹, DING Wenjuan¹, CHEN Yan ¹, PU Daojing ²

¹Department of Urology, ²Department of Infectious Diseases, Central Hospital of Enshi Autonomous Prefecture, Enshi 445000, Hubei, China

Received:2019-01-09 Revised:2019-02-12 Online:2019-06-26 Published:2019-06-25

摘要/Abstract

摘要：

目的:观察黄芪甲苷对人肾腺癌细胞ACHN增殖、凋亡的作用及其对SDF-1/CXCR4信号通路活化的影响。方法:采用20、60、120 μmol/L黄芪甲苷干预ACHN细胞后，CCK-8分析黄芪甲苷对细胞增殖抑制率的影响，流式细胞仪检测黄芪甲苷对细胞凋亡率的影响，Western Blot方法分析黄芪甲苷对细胞中凋亡相关蛋白Csapase-8、Bax、Bcl-2及SDF-1/CXCR4信号通路相关蛋白表达的影响。结果:黄芪甲苷干预ACHN细胞后，细胞增殖抑制率明显升高，表现出时间和剂量依赖性（P<0.05）；黄芪甲苷干预组ACHN细胞凋亡率也显著升高（P<0.05）；药物处理组中促凋亡蛋白Csapase-8、Bax表达显著升高，而抗凋亡蛋白Bcl-2及SDF-1、CXCR4蛋白表达水平显著降低，表现出剂量依赖性（P<0.05）。结论:黄芪甲苷抑制人肾腺癌细胞株ACHN增殖、诱导其凋亡的作用机制可能与降低SDF-1/CXCR4信号通路活化相关。

关键词: 黄芪甲苷, 人肾腺癌细胞, 增殖, 凋亡

Abstract:

AIM: To observe the effect of astragaloside on the proliferation, apoptosis of human renal carcinoma cell (ACHN) and the activation of SDF-1/CXCR4 signaling pathway. METHODS: ACHN cells were co-cultured with 20, 60 and 120 μmol/L of astragaloside. CCK-8 assay was used to detect the effect of astragaloside on proliferation inhibitory rates of ACHN cells. The apoptotic rates of ACHN cells were analyzed using flow cytometer. Western Blot was used to determine the expression levels of Csapase-8, Bax, Bcl-2, SDF-1 and CXCR4 proteins in ACHN cells. RESULTS:After treatment with 20, 60 and 120 μmol/L of astragaloside, the proliferation inhibitory rates of ACHN cells were significantly increased with time- and dose-dependent manner (P<0.05). The apoptotic rates of ACHN cells in astragaloside groups were significantly increased compared with control group (P<0.05). The expression levels of Csapase-8 and Bax in astragaloside groups were significantly increased, and the expression levels of Bcl-2, SDF-1 and CXCR4 were significantly decreased compared with control group (P<0.05). CONCLUSION: Astragaloside can inhibit the proliferation of ACHN cells and induce the apoptosis of ACHN cells, and its mechanism was associated with the inhibition of SDF-1/CXCR4 cascade.

Key words: astragaloside, human renal carcinoma cell, proliferation, apoptosis

中图分类号:

R736
R965.2

邹照银，郑府，徐先顺，丁文娟，陈艳，蒲道静. 黄芪甲苷通过SDF-1/CXCR4信号通路诱导人肾腺癌细胞的凋亡[J]. 中国临床药理学与治疗学, 2019, 24(6): 665-669.

ZOU Zhaoyin, ZHENG Fu, XU Xianshun, DING Wenjuan, CHEN Yan, PU Daojing. Effect of astragaloside on the apoptosis of human renal carcinoma cell (ACHN) via SDF-1/CXCR4 signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 665-669.

参考文献

［1］Cheng SK, Chuah KL. Metastatic renal cell carcinoma to the pancreas: a review［J］. Arch Pathol Lab Med, 2016, 140(6):598-602.
［2］Hes O, Compérat EM, Rioux-Leclercq N. Clear cell papillary renal cell carcinoma, renal angiomyoadenomatous tumor, and renal cell carcinoma with leiomyomatous stroma relationship of 3 types of renal tumors: a review［J］. Ann Diagn Pathol, 2016, 21(11):59-64.
［3］OuYang Y, Huang J, OuYang Z.Enrichment and purification process of astragalosides and their anti-human gastric cancer MKN-74 cell proliferation effect［J］. Afr Health Sci, 2014, 14(1):22-27.
［4］Cheng X, Gu J, Zhang M, et al. Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway［J］. Int Immunopharmacol, 2014, 23(1):304-313.
［5］Jiang K, Lu Q, Li Q, et al. Astragaloside IV inhibits breast cancer cell invasion by suppressing Vav3 mediated Rac1/MAPK signaling［J］. Int Immunopharmacol, 2017, 42(14):195-202.
［6］Teicher BA, Fricker SP. CXCL12(SDF-1)/CXCR4 pathway in cancer［J］.Clin Cancer Res, 2010, 16(11): 2927- 2931.
［7］Qin H, Liu P, Lin S. Effects of Astragaloside IV on the SDF-1/CXCR4 Expression in atherosclerosis of apoE(-/-) mice induced by hyperlipaemia［J］. Evid Based Complement Alternat Med, 2015, 385(11): 154-162.
［8］刘新辉. 黄芪甲苷对高糖诱导肾小管上皮细胞凋亡及线粒体自噬相关蛋白表达的影响［J］. 广州中医药大学学报, 2019, 36(2): 251-255.
［9］Lombardi L, Tavano F, Morelli F, et al. Chemokine receptor CXCR4: role in gastrointestinal cancer［J］. Crit Rev Oncol Hematol, 2013, 88 (3): 696-705.
［10］Wang L, Chen W, Gao L, et al. High expression of CXCR4, CXCR7 and SDF-1 predicts poor survival in renal cell carcinoma［J］. World J Surg Oncol, 2012, 10(5):212-219.
［11］Rasti A, Abolhasani M, Zanjani LS, et al. Reduced expression of CXCR4, a novel renal cancer stem cell marker, is associated with high-grade renal cell carcinoma［J］. J Cancer Res Clin Oncol, 2017, 143(1):95-104.
［12］Guo Q, Wu XH, Lu YP, et al. Relationship between chemokine axis CXCL12- CXCR4 and epithelial ovarian cancer［J］. Chin Med J, 2013, 93(21):1677-1680.
［13］Wu PF, Lu ZP, Cai BB, et al. Role of CXCL12/CXCR4 signaling axis in pancreatic cancer［J］. Chin Med J, 2013, 126(17): 3371-3374.
［14］Ni J, Xie X, Xie J, et al. Role of BCL-2,caspase-3 and NF-κB in astragaloside inducing apoptosis of human NB4 cells［J］. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2014, 22(3):703-706.
［15］Fan Y, Yang F, Cao X, et al. Gab1 regulates SDF-1-induced progression via inhibition of apoptosis pathway induced by PI3K/AKT/Bcl-2/BAX pathway in human chondrosarcoma［J］. Tumour Biol, 2016, 37(1):1141-1149.

[1]	范杰, 金永明, 江孝龙, 蒋国华. lncRNA HOTAIR调控miR-206影响类风湿关节炎滑膜细胞增殖和凋亡的分子机制研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 736-742.
[2]	王安婧, 王亚亚, 梁轩, 闫雅婕, 苏静, 李彩东. 基于PPAR治疗胆汁淤积性肝病的机制与药物研究进展[J]. 中国临床药理学与治疗学, 2023, 28(7): 796-808.
[3]	杨晓佳, 江萌, 吴敏, 张伊黎, 吕兰, 吴嫄芬, 王鑫昱, 刘立权. 藏红花素介导DKK3调控GSK-3β/β-catenin通路对阿尔兹海默症大鼠的认知改善作用[J]. 中国临床药理学与治疗学, 2023, 28(5): 489-497.
[4]	王艳, 张金辉, 赵永辰, 刘宏祥, 刘亚维, 苗欢欢, 杨信才. 红杉黄酮通过下调PI3K/AKT信号通路抑制胃癌细胞增殖侵袭等干细胞特性[J]. 中国临床药理学与治疗学, 2023, 28(5): 508-513.
[5]	侯小煜, 冷玉芳, 曹雪芬, 吕兴娇, 韩晓霞, Janvier NIBARUTA, 刘永强. 五味子乙素减轻小鼠肠缺血再灌注损伤的网络药理学分析及实验验证[J]. 中国临床药理学与治疗学, 2023, 28(2): 147-154.
[6]	王剑, 孙永东, 周兴玮, 刘雷, 陈龙, 童兴科, 朱佳丽. 黄芩素经由miR-125b-5p/IRF4轴进而促进喉癌细胞死亡并抑制其侵袭的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1209-1218.
[7]	朱瑞芳, 郭东凯, 智慧, 江翊国, 张悦翎, 钱晓萍, 季士亮. ROS-NF-κB-p38MAPK通路探索榄香烯联合硼替佐米抗多发性骨髓瘤的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1219-1226.
[8]	周欢, 徐铭, 李波, 刘春英, 王淳. PI3K/Akt/FOXO3a信号通路介导的保护性自噬参与肺腺癌获得性顺铂耐药表型重塑[J]. 中国临床药理学与治疗学, 2022, 27(9): 961-970.
[9]	华海燕, 严杰, 秦宇芬. 五味子乙素通过抑制心肌细胞凋亡减轻大鼠心肌缺血再灌注损伤的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(8): 848-856.
[10]	丁嘉敏, 王友娣, 赵夏丽, 姜根风, 陈思文, 洪程程, 李书勤, 胡卫华. 生长激素和维生素E联合应用治疗薄型子宫内膜的研究[J]. 中国临床药理学与治疗学, 2022, 27(8): 857-862.
[11]	李雪恒, 李龙. 褪黑素与特发性肺纤维化关系的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(6): 715-720.
[12]	王新丽, 许霞青, 方寒冰, 郭玉忠. 佛波酯增强顺铂的抗肿瘤活性及降低其肾毒性的作用研究[J]. 中国临床药理学与治疗学, 2022, 27(5): 535-543.
[13]	杨柳, 罗爱林, 李世勇. 全麻药物损伤发育期大脑的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(12): 1381-1390.
[14]	谭峰浪, 任宏丽, 王宏飞. 黄柏提取物对Aβ_1-42诱导的海马神经细胞的影响[J]. 中国临床药理学与治疗学, 2022, 27(11): 1213-1220.
[15]	李岩岩, 方骁杰, 尹鑫, 孙曦, 饶春晖. circZNF124通过靶向miR-4262调控结直肠癌SW620细胞增殖、迁移及侵袭的机制研究[J]. 中国临床药理学与治疗学, 2022, 27(11): 1231-1239.

黄芪甲苷通过SDF-1/CXCR4信号通路诱导人肾腺癌细胞的凋亡

Effect of astragaloside on the apoptosis of human renal carcinoma cell (ACHN) via SDF-1/CXCR4 signaling pathway

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价