天麻素对海马内Aβ1-40注射阿尔茨海默病模型大鼠学习记忆的改善作用

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (4): 408-411.

天麻素对海马内Aβ_1-40注射阿尔茨海默病模型大鼠学习记忆的改善作用

柳星, 汪萌芽

皖南医学院细胞电生理研究室,芜湖 241002,安徽

收稿日期:2011-12-05 修回日期:2012-02-17 发布日期:2012-04-28
通讯作者: 汪萌芽,男,博士,教授,硕士生导师,研究方向:细胞电生理与药理学。Tel: 0553-3932276 E-mail: wangmy@wnmc.edu.cn
作者简介:柳星,男,硕士研究生,研究方向:神经生理学。Tel: 15905533065 E-mail: xinglin@ahtcm.edu.cn
基金资助:
安徽省自然科学基金项目(090413084)

Improving effects of gastrodin on learning and memory in model rats with Alzheimer disease induced by intra-hippocampal Aβ_1-40 injection

LIU Xing, WANG Meng-ya

Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu 241002, Anhui, China

Received:2011-12-05 Revised:2012-02-17 Published:2012-04-28

摘要/Abstract

摘要： 目的: 观察天麻素对海马内β-淀粉样蛋白1-40(Aβ_1-40)注射阿尔茨海默病(Alzheimer disease, AD)模型大鼠空间学习记忆能力的影响。方法: 双侧海马内注射Aβ_1-40建立AD大鼠模型, 并设注射生理盐水的假手术组(n=7)和不作处理的正常组(n=10);AD模型大鼠分设天麻素组、石杉碱甲组和模型组(各n=8),分别给予药物或生理盐水灌胃4周(q.d.)后,用Morris水迷宫进行学习记忆能力检测。结果: 与正常组或假手术组相比,模型组大鼠在Morris水迷宫的定位航行试验中逃避潜伏期明显延长(P<0.01 或P<0.05)。与模型组比较,天麻素组大鼠的逃避潜伏期明显缩短(P<0.05),且空间探索试验中在原平台所在象限停留时间明显延长(P<0.05)。结论: 天麻素对海马内Aβ_1-40注射AD模型大鼠的学习记忆损害有一定的改善作用。

关键词: 天麻素, 阿尔茨海默病, 动物模型, 迷宫学习

Abstract: AIM: To observe the effects of gastrodin treatment on learning and memory of model rats with Alzheimer disease (AD) induced by intra-hippocampal Aβ_1-40 injection. METHODS: The AD rat model was established by injection of Aβ_1-40 into bilateral hippocampus, rats in sham group (n=7) was injected with saline, and normal group (n=10) without any treatment. The AD model rats in gastrodin group, huperzine A group and model group (n=8 each group), were administered by oral gavage of drugs or saline, respectively, for 4 weeks (q.d.). Then Morris water maze was used to examine the changes of learning and memory abilities in rats. RESULTS: The test of Morris water maze showed that the escape latency of place navigation trials in model group was significantly longer than normal or sham groups (P<0.01 or P<0.05). In comparison with model group, the escape latency in gastrodin group was significantly shorter (P<0.05), but the time in presumable plate quadrant of spatial probe trial was longer (P<0.05). CONCLUSION: Gastrodin may have improving effect on the abilities of learning and memory of the model rats with AD induced by intra-hippocampal Aβ_1-40 injection.

Key words: Gastrodin, Alzheimer disease, Animal models, Maze learning

中图分类号:

R965.2

柳星, 汪萌芽. 天麻素对海马内Aβ_1-40注射阿尔茨海默病模型大鼠学习记忆的改善作用[J]. 中国临床药理学与治疗学, 2012, 17(4): 408-411.

LIU Xing, WANG Meng-ya. Improving effects of gastrodin on learning and memory in model rats with Alzheimer disease induced by intra-hippocampal Aβ_1-40 injection[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 408-411.

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