姜黄素对Lewis肺癌小鼠血管内皮成长因子和endostatin表达的影响

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (2): 151-154.

姜黄素对Lewis肺癌小鼠血管内皮成长因子和endostatin表达的影响

徐步远¹，潘显阁¹，陈崇别¹，蔡敏¹，徐露²

¹温州市平阳县人民医院，平阳 325400 ，浙江；²重庆医科大学药学院，重庆 400016

收稿日期:2016-11-09 修回日期:2017-01-04 出版日期:2017-02-26 发布日期:2017-03-02
作者简介:徐步远, 男，副主任医师，副教授，研究方向：胸外科，微创外科。 Tel：13758820707 E-mail:41903669@qq.com

Effects of curcumin on VEGF and endostatin expression of Lewis lung carcinoma

XU Buyuan¹, PAN Xiange¹, CHEN Chongbie¹, CAI Min ¹, XU Lu ²

¹People's Hospital of Pingyang County of Wenzhou, Pingyang 325400, Zhejiang, China; ² College of Pharmacy, Chongqing Medical University, Chongqing 400016, China

Received:2016-11-09 Revised:2017-01-04 Online:2017-02-26 Published:2017-03-02

摘要/Abstract

摘要：

目的:探讨姜黄素对Lewis肺癌组织中血管内皮成长因子（VEGF）和血管生成抑制因子抑制素（endostatin）表达的影响。方法: 本实验分为3组：空白对照组、模型组、姜黄素组（100 mg/kg）。空白对照组设10只C57BL/6纯系小鼠，另20只小鼠右腋皮下接种Lewis瘤细胞，随机分为2组：模型组、姜黄素组（100 mg/kg），每组10只。接种成功后，空白对照组与模型组灌胃0.5%羟甲基纤维素钠溶液，姜黄素组灌胃100 mg/kg，灌胃容积为0.2 mL/10 g，连续灌胃14 d，每天1次。末次给药后处死小鼠，称其体重，剥离肿瘤组织称重后采用Western Blot法和Q-PCR法检测VEGF、endostatin蛋白及基因的表达。结果:给予Lewis肺癌小鼠姜黄素治疗后肿瘤体积与质量明显减少，VEGF的表达显著减少和endostatin的表达明显增加。结论:姜黄素抑制肺癌肿瘤的生长，其作用可能是通过下调VEGF与上调endostatin的表达，从而抑制肿瘤血管的生长。

关键词: 姜黄素, 血管内皮成长因子, endostatin

Abstract:

AIM: To investigate the effects of curcumin on vascular endothelial growth factor (VEGF) and endostatin expression in lung tumor tissues isolated from Lewis lung carcinoma. METHODS: Three groups were established: the control, the tumor model and the curcumin treatment. Ten C57BL/6 mice were assigned into the control group. Another 20 mice were subcutaneously injected with Lewis cells into the right limb armpit and were randomly divided into the model group and the curcumin group, ten in each. 20 mice of control and model groups were then administered intragastrically with 0.5% CMC-Na solution for 14 days, while mice of the treatment group received suspension of curcumin (100 mg/kg) solution by gavage for 14 days. Lung tumor tissues were isolated from each group after final administration. Weights of the mice and Lung tumor tissues were measured. VEGF and endostatin expression were analyzed by Western blot and Q-PCR. RESULTS: Weigts of lung tumor tissues reduced significantly. VEGF expression increased in lung tumor tissues, while endostatin expression decreased. CONCLUSION: Curcumin inhibits the development of lung tumor through down-regulating VEGF expression and up-regulating endostatin expression.

Key words: curcumin, vascular endothelial growth factor, endostatin

中图分类号:

R965.2

徐步远，潘显阁，陈崇别，蔡敏，徐露. 姜黄素对Lewis肺癌小鼠血管内皮成长因子和endostatin表达的影响[J]. 中国临床药理学与治疗学, 2017, 22(2): 151-154.

XU Buyuan, PAN Xiange, CHEN Chongbie, CAI Min, XU Lu. Effects of curcumin on VEGF and endostatin expression of Lewis lung carcinoma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(2): 151-154.

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