姜黄素对大鼠子宫内膜异位症影响的实验研究#br#

doi:10.12092/j.issn.1009-2501.2020.08.004

中国临床药理学与治疗学 ›› 2020, Vol. 25 ›› Issue (8): 862-868.doi: 10.12092/j.issn.1009-2501.2020.08.004

姜黄素对大鼠子宫内膜异位症影响的实验研究#br#

沈龙德¹，李爱禄¹，李丽春¹，吴银银²，沈龙源³，江秋霞⁴，贺锦曦¹，吴志芬¹，余黎吉¹，张晓红¹

¹福建医科大学附属泉州第一医院妇产科，泉州 362000，福建；²晋江市中医院妇产科，泉州 362200，福建；³泉州市妇幼保健院·儿童医院麻醉科，泉州 362000，福建；⁴泉州市妇幼保健院·儿童医院超声科，泉州 362000，福建

收稿日期:2019-11-18 修回日期:2020-07-29 出版日期:2020-08-26 发布日期:2020-09-03
作者简介:沈龙德，男，硕士，主治医师，研究方向：妇科。 Tel: 13655998388 E-mail: sld123321@126.com
基金资助:
泉州市科技计划（2018Z058）

Experimental study on the effect of curcumin on endometriosis in rats

SHEN Longde ¹, LI Ailu ¹, LI Lichun ¹, WU Yinyin², SHEN Longyuan ³, JIANG Qiuxia⁴, HE Jinxi¹, WU Zhifen¹, YU Liji¹, ZHANG Xiaohong¹

¹ Obstetrics and Gynecology Department, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou 362000, Fujian, China; ² Obstetrics and Gynecology Department, Jinjiang Traditional Chinese Medicine Hospital, Jinjiang 362200, Fujian, China; ³ Department of Anesthesiology, Quanzhou Women's and Children's Hospital, Quanzhou 362000, Fujian, China; ⁴ Ultrasonography Department, Quanzhou Women's and Children's Hospital, Quanzhou 362000, Fujian, China

Received:2019-11-18 Revised:2020-07-29 Online:2020-08-26 Published:2020-09-03

摘要/Abstract

摘要： 目的：分析姜黄素对大鼠子宫内膜异位组织转录因子Twist1、Twist2、E-钙黏蛋白（E-cadherin）、Syndecan-1表达的影响。方法：选取SPF级雌性SD大鼠采用自体内膜移植法建立子宫内膜异位症大鼠模型，将造模成功的30只大鼠随机分为模型组、低剂量姜黄素组（25 mg·kg-1·d-1）、中剂量姜黄素组（50 mg·kg-1·d-1）、高剂量姜黄素组（100 mg·kg-1·d-1）、孕三烯酮组（0.5 mg·kg-1·d-1），每组各6只，设立5只大鼠假手术组为空白对照组，记录各组大鼠异位内膜组织体积；采用免疫组化法检测各组大鼠子宫内膜异位组织Twist1、Twist2、E-cadherin、Syndecan-1表达的变化。结果：与模型组相比，姜黄素各剂量组和孕三烯酮组大鼠异位组织体积明显较低，差异具有统计学意义（P<0.05）；Twist1阳性主要定位于细胞核中，Twist2阳性主要定位于细胞质中，E-cadherin、Syndecan-1阳性染色主要定位于上皮细胞膜上，少见于个别细胞质。与模型组相比，姜黄素各剂量组和孕三烯酮组Twist1、Twist2、Syndecan-1评分均明显降低，E-cadherin评分明显升高（P<0.05），低中剂量姜黄素组Twist1、Twist2、Syndecan-1评分明显高于孕三烯酮组，E-cadherin评分低于孕三烯酮组（P<0.05），高剂量姜黄素组Twist1、Twist2、E-cadherin、Syndecan-1评分与孕三烯酮组比较差异无统计学意义（P>0.05）。结论：姜黄素可降低子宫内膜异位组织中Twist1、Twist2、Syndecan-1的表达，升高E-cadherin的表达，抑制模型大鼠异位内膜的生长，以达到治疗的目的。

关键词: 姜黄素, 子宫内膜异位, Twist1, Twist2, E-cadherin, Syndecan-1

Abstract: AIM: To analyze the effect of curcumin on the expression of transcription factors Twist1, Twist2, E-cadherin and Syndecan-1 in rats with endometriosis. METHODS: Thirty SD rats were randomly divided into five groups: model group, low dose curcumin group (25 mg·kg-1·d-1), medium dose curcumin group (50 mg·kg-1·d-1), high dose curcumin group (100 mg·kg-1·d-1) and gestrinone group (0.5 mg·kg-1·d-1). There were 6 rats in each group, and 5 rats in sham operation group were set up as blank control group. The expression of Twist1, Twist2, E-cadherin and Syndecan-1 was detected by immunohistochemistry. RESULTS: Compared with the model group, the ectopic tissue volume of curcumin group and gestrinone group was significantly lower, the difference was statistically significant (P<0.05). Positive Twist1 was mainly located in the nucleus, positive Twist2 was mainly located in the cytoplasm, positive E-cadherin and Syndecan-1 staining was mainly located in the epithelial cell membrane, rarely in individual cytoplasm. Compared with the model group, the scores of Twist1, Twist2 and Syndecan-1 in each dose group of curcumin and gestrinone group were significantly lower, and the scores of Twist1, Twist2 and Syndecan-1 in low and middle dose curcumin group were significantly higher than those in gestrinone group, and the scores of E-cadherin were lower than those in gestrinone group (P<0.05). The scores of Twist1, Twist2, E-cadherin and Syndecan-1 in high dose curcumin group were significantly higher. There was no significant difference between the high dose curcumin group and gestrinone group (P>0.05). CONCLUSION: Curcumin can reduce the expression of Twist1, Twist2 and Syndecan-1, increase the expression of E-cadherin, and inhibit the growth of endometrium in the model rats, so as to achieve the purpose of treatment.

Key words: curcumin, endometriosis, Twist1, Twist2, E-cadherin, Syndecan-1

中图分类号:

沈龙德, 李爱禄, 李丽春, 吴银银, 沈龙源, 江秋霞, 贺锦曦, 吴志芬, 余黎吉, 张晓红. 姜黄素对大鼠子宫内膜异位症影响的实验研究#br#[J]. 中国临床药理学与治疗学, 2020, 25(8): 862-868.

SHEN Longde, LI Ailu, LI Lichun, WU Yinyin, SHEN Longyuan, JIANG Qiuxia, HE Jinxi, WU Zhifen, YU Liji, ZHANG Xiaohong. Experimental study on the effect of curcumin on endometriosis in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 862-868.

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姜黄素对大鼠子宫内膜异位症影响的实验研究#br#

Experimental study on the effect of curcumin on endometriosis in rats

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