辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响

doi:10.12092/j.issn.1009-2501.2018.03.008

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (3): 290-295.doi: 10.12092/j.issn.1009-2501.2018.03.008

辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响

吕思敏¹，黄志荣²，于琼²，孙金影²，崔燎³，吴铁^1,2

¹广东医科大学·广东润和生物科技有限公司辅酶Q10联合研究中心，东莞 523808，广东；²广东医科大学药学院，东莞 523808，广东；³广东天然药物研究与开发重点实验室，湛江 524023，广东

收稿日期:2017-06-26 修回日期:2018-02-14 出版日期:2018-03-26 发布日期:2018-03-28
通讯作者: 吴铁，男，硕士，教授，研究方向：抗炎免疫与骨质疏松药理学。 Tel：0769-22896560 E-mail：wutie2@163.com
作者简介:吕思敏，女，硕士，研究方向：骨药理学。 E-mail：lvsimin88@126.com
基金资助:
广东医科大学·广东润和生物科技有限公司共建辅酶Q10联合研究中心项目（2XX14002）

Effects of coenzyme Q10 chaperone drinks on femur and ulna in the mild aging mice

LV Simin ¹, HUANG Zhirong ², YU Qiong ², SUN Jinying², CUI Liao ³, WU Tie ^1,2

¹ Guangdong Medical University & Guangdong Runhe Biological Technology Co., Ltd, Coenzyme Q10 Research Institute, Dongguan 523808, Guangdong, China; ²School of Pharmacy, Guangdong Medical University, Dongguan 523808, Guangdong, China; ³ Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, Guangdong, China

Received:2017-06-26 Revised:2018-02-14 Online:2018-03-26 Published:2018-03-28

摘要/Abstract

摘要：

目的:应用Micro CT技术和骨成分分析方法，观察辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响。方法: 8月龄SPF级昆明小鼠，♀♂兼用，随机分为生理盐水组（NS），辅酶Q10组（Q10），辅酶Q10伴侣饮料组（QC），持续给药10周。取右侧股骨用于Micro CT扫描，同时取左侧尺骨进行骨成分测定。结果: 实验结束时，QC组小鼠体质量比实验前降低；与NS组相比，QC组羟脯氨酸（Hyp）含量显著降低，骨钙和骨镁含量均显著增加，骨体积分数（BV/TV）明显增加，骨密度（Density）和连接密度（Conn-Dens）均显著增加，结构模型指数（SMI）显著降低；与Q10组相比，QC组Hyp含量明显降低，骨钙和骨镁含量均显著增加；Micro CT三维图显示QC组骨小梁明显增粗，数目增多，紧密程度较好，连续性得到恢复，较NS组在小梁骨的数量、厚度以及分布上均有明显改善。结论:辅酶Q10及其伴侣饮料可以增加轻度衰老期小鼠股骨的骨量，微观结构得到改善，具有良好的抗骨质疏松应用前景。

关键词: 辅酶Q10, 轻度衰老期, Micro CT, 骨成分分析

Abstract:

AIM: To investigate the effect of coenzyme Q10 chaperone drinks on femur and ulna in the mild aging mice with Micro CT technology and bone composition analysis method. METHODS: Eight-month-old SPF grade Kunming mice, both male and female, were randomly into normal saline group (NS), coenzyme Q10 Group (Q10), coenzyme Q10 chaperone drinks group (QC). After 10 weeks, the right femur was performed on Micro CT, and the left ulna bone was collected for detecting the content of Ca, Mg and Hyp. RESULTS: After giving coenzyme Q10 chaperone drinks, the body weight decreased in mice. Compared with NS group, QC group had a significant decrease in the Hyp content, SMI, and increased significantly in Ca, Mg content, BV/TV, Density and Conn-Dens. Compared with Q10 group, QC group had a significant decrease in the Hyp content, and increased significantly in Ca, Mg content. Micro CT three-dimensional image showed that the trabecular bone increased significanty, the number increased, and the degree of tightness was better, the continuity was restored in QC group, while compared with the NS group, the trabecular bone quantity, thickness, and distribution were significantly improved. CONCLUSION: Coenzyme Q10 chaperone drinks can improve the bone mass in the mild aging mice, suggesting a good prospects of the components in anti-osteoporosis application.

Key words: coenzyme Q10, mild aging mice, Micro CT, bone composition analysis

中图分类号:

R965.2

吕思敏，黄志荣，于琼，孙金影，崔燎，吴铁. 辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响[J]. 中国临床药理学与治疗学, 2018, 23(3): 290-295.

LV Simin, HUANG Zhirong, YU Qiong, SUN Jinying, CUI Liao, WU Tie. Effects of coenzyme Q10 chaperone drinks on femur and ulna in the mild aging mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(3): 290-295.

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辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响

Effects of coenzyme Q10 chaperone drinks on femur and ulna in the mild aging mice

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