新霉素损伤哺乳动物前庭毛细胞后的转录组学变化分析

doi:10.12092/j.issn.1009-2501.2019.05.001

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (5): 481-489.doi: 10.12092/j.issn.1009-2501.2019.05.001

• 基础研究 • 下一篇

新霉素损伤哺乳动物前庭毛细胞后的转录组学变化分析

韩京宏¹，刘宇辰²，聂国辉¹

¹北京大学深圳医院耳鼻喉科，深圳 518000，广东； ²深圳市第二人民医院转化医学研究院，深圳 518000，广东

收稿日期:2019-02-26 修回日期:2019-04-30 出版日期:2019-05-26 发布日期:2019-05-28
通讯作者: 聂国辉，男，博士，教授，研究方向：内耳毛细胞再生。 Tel：13823227303 E-mail：nghui@21cn.com
作者简介:韩京宏，女，硕士研究生，研究方向：内耳毛细胞再生。 Tel：18801232513 E-mail：972573393@qq.com
基金资助:
国家自然科学基金青年基金项目（81402103）；深圳市发改委、深圳眩晕诊治与前庭功能重塑公共服务平台项目，深发改【2017】436号（s2016005470013）

Transcriptomic analysis of neonatal mammalian vestibular after hair cell injury by neomycin

HAN Jinghong¹, LIU Yuchen², NIE Guohui¹

¹ Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen 518000, Guangdong, China; ²Institute of Translational Medicine, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen 518000, Guangdong, China

Received:2019-02-26 Revised:2019-04-30 Online:2019-05-26 Published:2019-05-28

摘要/Abstract

摘要：

目的: 探讨毛细胞损伤过程中的基因表达、信号通路、生物学过程变化，为毛细胞再生及前庭功能重塑提供新的思路。方法: 采用RNA-seq方法研究新生小鼠椭圆囊在新霉素损伤后的基因表达变化及相关分子通路。结果: 新霉素处理样品中分别有172个和232个基因表达上调和下调。这些基因的GO和KEGG通路分析揭示了许多可能与毛细胞发育相关的细胞成分、分子功能、生物过程和信号通路。结论: 本研究的结果为未来的研究提供了有价值的参考和前期基础，并发现Wnt、Notch和TGFβ等信号通路可能与前庭毛细胞损伤及再生密切相关，可能为前庭毛细胞再生研究及临床眩晕防治提供重要信号通路和基因靶点。

关键词: 前庭毛细胞, 新霉素, RNA-seq

Abstract:

AIM: Vestibular hair cell damage is an important cause of vestibular dysfunction. This study tried to explore the changes of gene expression, signal pathway and biological process in the process of hair cell damage, so as to provide new ideas for hair cell regeneration and vestibular function recovery. METHODS: In the present study, RNA from the newborn mouse utricles was collected for RNA-seq immediately after the damage of neomycin, in order to investigate gene expression changes. RESULTS:There were 172 and 232 genes that were found to be up-regulated and down-regulated in neomycin-treated utricles. GO and KEGG pathway analyses of these genes revealed many deregulated cellular components, molecular functions, biological processes and signaling pathways that may be related to hair cell development.CONCLUSION: These results provide valuable reference and preliminary basis for future studies, and find that Wnt, Notch TGFβ and other signaling pathways that may be closely related to vestibular hair cell injury and regeneration, which may provide important signaling pathways and gene targets for vestibular hair cell regeneration research and clinical vertigo prevention.

Key words: vestibular hair cell, neomycin, RNA-seq

中图分类号:

R966

韩京宏，刘宇辰，聂国辉. 新霉素损伤哺乳动物前庭毛细胞后的转录组学变化分析[J]. 中国临床药理学与治疗学, 2019, 24(5): 481-489.

HAN Jinghong, LIU Yuchen, NIE Guohui. Transcriptomic analysis of neonatal mammalian vestibular after hair cell injury by neomycin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 481-489.

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新霉素损伤哺乳动物前庭毛细胞后的转录组学变化分析

Transcriptomic analysis of neonatal mammalian vestibular after hair cell injury by neomycin

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