复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响

doi:10.12092/j.issn.1009-2501.2019.12.008

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (12): 1364-1370.doi: 10.12092/j.issn.1009-2501.2019.12.008

复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响

李弘，刘叶倩，龚姗，刘林，曾勇，蔡晓，王宇红，任卫琼

湖南中医药大学第一附属医院，长沙 410007，湖南；湖南中医药大学科技创新中心，长沙 410208，湖南

收稿日期:2019-07-01 修回日期:2019-12-22 出版日期:2019-12-26 发布日期:2020-01-07
通讯作者: 任卫琼，硕士，副主任药师，研究方向：心血管疾病的中医药防治。 Tel：13037311165 E-mail：renweiqiong1@126.com
作者简介:李弘，在读硕士，研究方向：心血管疾病的中医药防治。 Tel：15855401223 E-mail：1006064773@qq.com
基金资助:
湖南省中医药管理局重点项目（201910）；湖南省科技厅项目（2018SK51203）；中医内科重大疾病防治及转化教育部重点实验室开放基金（ZYNK201703）；研究生校级创新课题（2018CX37）；国家自然科学基金（81473616）

Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type III collagen

LI Hong, LIU Yeqian, GONG Shan, LIU Lin, ZENG Yong, CAI Xiao, WANG Yuhong, REN Weiqiong

The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, China; Innovation Centre for Science and Technology, Changsha 410208, Hunan, China

Received:2019-07-01 Revised:2019-12-22 Online:2019-12-26 Published:2020-01-07

摘要/Abstract

摘要：

目的:探究复方七芍降压片对自发性高血压大鼠（SHR）血压及血管重塑的作用机制。方法:SHR大鼠随机分为模型组、厄贝沙坦片组、复方七芍降压片组，每组10只，另设10只WKY大鼠为正常对照组，给药6周，无创血压仪测量各周大鼠血压变化；HE染色观察大鼠肠系膜动脉病理变化并测量管壁厚度、血管中膜厚度及相同位置的管腔直径，比值记为WT；免疫组化法检测肠系膜动脉中基质金属蛋白酶-2（MMP-2）及Ⅰ、Ⅲ型胶原蛋白（COⅠ、COⅢ）表达情况。结果:给药6周后，与正常对照组比较，模型组大鼠各周血压均明显升高（P<0.01），管壁厚度及WT值增高（P<0.01或P<0.05），肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显升高（P<0.01）；与模型组比较，复方七芍降压片组大鼠各周血压均明显降低（P<0.01），管壁厚度及WT值降低（P<0.05），肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显降低（P<0.05），且病理变化得到改善。结论:复方七芍降压片具有降低SHR大鼠血压，改善血管重塑的作用，其机制可能与降低MMP-2表达及Ⅰ、Ⅲ型胶原蛋白沉积有关。

关键词: 复方七芍降压片, 肠系膜动脉, 基质金属蛋白酶-2, I型胶原蛋白, Ⅲ型胶原蛋白, 血管重塑

Abstract:

AIM: To explore the mechanism of compound Qishao Jiangya tablet on blood pressure and vascular remodeling in spontaneously hypertensive rats (SHR). METHODS: SHR rats were randomly divided into model group, irbesartan tablet group and compound Qishao Jiangya tablet group, 10 rats in each group; Another 10 WKY rats were used as normal control group; All received administration for 6 weeks; noninvasive sphygmomanometer was used to measure the changes of blood pressure in rats; HE staining was used to observe the pathological changes of mesenteric artery in rats and to measure the thickness of mesenteric artery wall; The thickness of intima media and the diameter of lumen in the same position were recorded as WT; Detection of matrix metalloprotease-2 (MMP-2) and type Ⅰ and type III collagen (CO Ⅰ, CO III) protein expression in mesenteric artery was performed by immuno-histochemical method. RESULTS:After 6 weeks of administration, compared with the normal control group, the blood pressure of the model group was significantly higher than that of the control group (P<0.01), and the thickness of tube wall and WT value were increased (P<0.01 or P<0.05), the expression of MMP -2, I and III collagen in mesenteric artery increased significantly (P<0.01); Compared with model group, the blood pressure of the rats in the compound Qishao Jiangya tablet group was significantly lower than that in the control group (P<0.01), The thickness of tube wall and WT value decreased (P<0.05), the expression of MMP-2 and type Ⅰ and type III collagen in mesenteric artery was significantly decreased (P<0.05), and the pathological changes were improved. CONCLUSION: Compound Qishaojiang tablet can reduce blood pressure and improve vascular remodeling in SHR rats, the mechanism may be related to the decrease of MMP-2 expression and the deposition of type Ⅰ and type III collagen.

Key words: compound Qishao Jiangya tablet, mesenteric artery, matrix metalloprotease-2, type Ⅰ collagen, type III collagen, vascular remodeling

中图分类号:

R965.2
R544

李弘，刘叶倩，龚姗，刘林，曾勇，蔡晓，王宇红，任卫琼. 复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响[J]. 中国临床药理学与治疗学, 2019, 24(12): 1364-1370.

LI Hong, LIU Yeqian, GONG Shan, LIU Lin, ZENG Yong, CAI Xiao, WANG Yuhong, REN Weiqiong. Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type III collagen[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1364-1370.

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复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响

Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type III collagen

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