猪苓多糖和水飞蓟宾对CC4中毒肝细胞膜超微结构的保护作用

中国临床药理学与治疗学 ›› 1996, Vol. 1 ›› Issue (2): 81-83.

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猪苓多糖和水飞蓟宾对CC₄中毒肝细胞膜超微结构的保护作用

汪茂荣, 乐美兆, 许家璋, 何长伦, 夏廷玉

解放军第81医院传染病中心,南京210002

收稿日期:1996-07-01 修回日期:1996-08-23 发布日期:2020-12-03
作者简介:汪茂荣,男,34岁,硕士,许家璋,男, 54岁,主任医师,教授,全军传染病专业学术委员会副主任委员。
基金资助:
本课题由全军“八·五”科研攻关青年基金资助。

Polyporus Umbellalus Ploysaccharides and Silybin in protecting ultrostructure of human fetal hepatocytes injured by CCL₄

Wang Maorong, Le Meizhao, Xu Jiazhang, et al

The Department of Infectious Disease, 81 Hospital, Nanjing 2100002

Received:1996-07-01 Revised:1996-08-23 Published:2020-12-03

摘要/Abstract

摘要： 目的应用人胎肝细胞CCL中毒模型,研究保肝药物猪苓多糖和水飞蓟宾对人胎肝细胞超微结构的保护作用。方法肝细胞来源于 5~7月龄水囊引产胎儿,通过胶原酶消化分离及培养,分别与猪苓多糖和水飞蓟宾作用不同时间后,再进行CCL中毒。通过检测培养上清中转氣酶和SOD的变化和观察扫描电镜及透射电镜镜下肝细胞形态及超微结构的改变来判断药物对肝细胞的作用。结果经与猪苓多糖和水飞蓟宾作用后的肝细胞,CCL中毒后其培养上清中ALT和AST水平明显较对照组减低, SOD水平增高。扫描电镜和透射电镜均表明,猪苓多糖和水飞蓟宾组肝细胞损伤明显减轻。在相同剂量下,前者对培养.上清中ALT、AST的降低及细胞膜表面超微结构的保护作用优于后者。结论猪苓多糖和水飞蓟宾对肝细胞膜表面超微结构均有-定的保护作用,可能是通过清除氧自由基而实现的。

关键词: 猪苓多糖, 水飞蓟宾, 胎肝细胞

Abstract: Aim In order to investigate the protective effects of Silybin and Polyporus Umbellalus Polysaccharides (PSP) on the ultrostructure of human hepatocytes a new experimental model system was founded by human fetal hepatocytes injued by CCL. Methods The hepatocytes the liver of human fetus aged. between 5 and 7 monthes were pretreated by collegenase digestion, treated with Silybin and PSP for different times and then exposed to CCL for 4 hours. Under scanning electron microscope (SEM) the hepatocytes were observed and the transaminase. and SOD in the culture medium were also assayed at the end of culture. Results The levels of transaminaese ALT and AST were largely decresed and the SOD level was elevated in culture medium in two medicines groups when compared with that in control group. And also the cellular integrity and membrane ultrostructure of hepatocytes were well preserved in two drugs groups. Conclusion Polyporus Um bellalus Ploysaccharides (PSP) and Silybin are good drugs for treatment of the liver damage. This model system founded by human fetal hepato-cytes injured by CCL could acl as one of useful methods for observing the effects of drugs on human hepatocytes.

Key words: Polyporus umbellalus polysaccharides(PSP), Silybin, Human fetal hepatocytes

中图分类号:

R975.5

汪茂荣, 乐美兆, 许家璋, 何长伦, 夏廷玉. 猪苓多糖和水飞蓟宾对CC₄中毒肝细胞膜超微结构的保护作用[J]. 中国临床药理学与治疗学, 1996, 1(2): 81-83.

Wang Maorong, Le Meizhao, Xu Jiazhang, et al. Polyporus Umbellalus Ploysaccharides and Silybin in protecting ultrostructure of human fetal hepatocytes injured by CCL₄[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 1996, 1(2): 81-83.

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猪苓多糖和水飞蓟宾对CC₄中毒肝细胞膜超微结构的保护作用

Polyporus Umbellalus Ploysaccharides and Silybin in protecting ultrostructure of human fetal hepatocytes injured by CCL₄

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