H-2 Ag-TCS 连接物制备及抑制异种脾细胞增殖活性的研究*

中国临床药理学与治疗学 ›› 1997, Vol. 2 ›› Issue (3): 174-176.

H-2 Ag-TCS 连接物制备及抑制异种脾细胞增殖活性的研究^*

兰平, 杨志勇¹, 王吉甫²

华西医科大学附属第一医院, 成都 6100411 重庆医科大学附属第二医院;
²中山医科大学附属第一医院

收稿日期:1997-05-12 修回日期:1997-06-02 出版日期:1997-09-26 发布日期:2020-12-04
作者简介:兰平,男33,博士后,从事器官移植免疫耐受的诱导。王吉甫,男,70岁,全国胃肠外科协会主席。
基金资助:
*国家自然科学基金资助

Preperation of H-2 Ag-TCS conjugate and inhibition of proliferation of recipientspleen cell in vitro

Lan Ping, Yang Zhiyong, Wang Jiepu

Department of Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041

Received:1997-05-12 Revised:1997-06-02 Online:1997-09-26 Published:2020-12-04

摘要/Abstract

摘要： 目的诱导特异性免疫无反应, 防止器官移植排斥反应。方法供者(NIH 小鼠)主要组织相容性抗原(H-2 Ag)与天花粉毒素(TCS)经异型双功能试剂SPDP、2-IT 交联, 制备免疫毒素(AG^-TCS);AG^-TCS 预处理受者(SD 大鼠), 体外观察其脾细胞对供者抗原再刺激的反应。结果受者脾细胞对供者抗原再刺激呈抑制状态, 而对非特异性刺激(PHA)呈增生活跃。结论 AG^-TCS 诱导的免疫无反应性具有供者特异性, 可用于器官移植免疫耐受的诱导。

关键词: H-2 抗原, 免疫毒素, 免疫无反应性

Abstract: Aim Specific immunologic unresponsiveness was induced to provent rejection of org an transplantation.Methods Donor (NIH mouse)H-2 antigen and trichosanthin (TCS)was conjugated to immunotox in (AG^-TCS)by hetero bifunctional reagent SPDP、2-it.The recipient (SD rat)was pretreated by AG^-TCS.In vitro the proliferation of the spleen cell, which was stimulated by donor spleen cell was observed.Results The proliferation of the SD ratspleen cell in vitro was inhibited when stimula ted by donor antigen, but it was activited by PHA.Conclutions The immunologic unresponsivness could be induced by AG^-TCS, which was useful in inducement of transplantation to lerance.

Key words: H-2 antigen, immunotoxin, immunol unresponsivness

中图分类号:

R967

兰平, 杨志勇, 王吉甫. H-2 Ag-TCS 连接物制备及抑制异种脾细胞增殖活性的研究^*[J]. 中国临床药理学与治疗学, 1997, 2(3): 174-176.

Lan Ping, Yang Zhiyong, Wang Jiepu. Preperation of H-2 Ag-TCS conjugate and inhibition of proliferation of recipientspleen cell in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 1997, 2(3): 174-176.