自动化微生物比浊法测定血清中环丙沙星浓度

中国临床药理学与治疗学 ›› 1998, Vol. 3 ›› Issue (3): 190-191.

自动化微生物比浊法测定血清中环丙沙星浓度

陈迁, 王睿, 方翼

解放军总医院, 北京100853

收稿日期:1998-04-20 发布日期:2020-12-02
作者简介:陈迁, 男, 30 岁, 药理学硕士, 主要研究方向:抗菌药物临床药理学。

Microbiological turbidimetric method in determination of serum concentrations of ciprofloxaxin

CHEN Qiang, WANG Rui, FANG Yi

Dept of Clinical Pharmacology, Chinese PL A General Hospital, Beijing 100853

Received:1998-04-20 Published:2020-12-02

摘要/Abstract

摘要： 目的建立自动化微生物比浊法测定血清环丙沙星(CPLX)浓度。方法以标准血清配制系列浓度CPLX 与10⁷ cfu/ml 标准菌ATCC 27853 反应, 在自动化微生物分析仪中测定其吸收度。结果在8 ~ 0.0625 μg /ml 浓度范围内, 浓度与吸收度呈线性关系(r =0.9950, n =8), 回归方程为C =9.4020 -22.55 A, 最低检测浓度为0.03 μg /ml 。方法回归率为96.5 % ~ 108.0 %, 日内变异系数为2.11% ~ 9.13 %, 日间变异系数为1.99 % ~ 9.72 %。结论该方法为一快速、方便、精确的测定CPLX 的方法。

关键词: 微生物比浊法, 环丙沙星

Abstract: Aim The application of microbiological turbidimetric method to determination of serum concentrations (C) of ciprofloxacin (CPLX) was studied. Methods A series diluted concentrations of CPLX were prepared with blank serum. The solution was put in a research unit with standard bacteria (ATCC 27853) dilution of 10⁷ cfu/ ml and incubated in Avantage analysis system. Absorbance (A) was recorded once every 5 min in a wavelength of 670 nm. Results There was a liner relationship (r =0.9950, n =8) between concentrations of CP LX and absorbance. The regression equation was C =9.4020-22.55 A. The lowest detectable concentration was 0.03 μg /ml. The rate of recovery of the method was 96.5 % ~ 108.0 %. The coefficient of variation within a day was 2.11% ~ 9.13 % while the coefficient of variation in different days was 1.99 % ~ 9.72 %. Conclusion The microbiological turbidimetry is a rapid, convenient and accurate method to determine the concentration of CPLX.

Key words: microbiological turbidity method, ciprofloxacin

中图分类号:

R978.1

陈迁, 王睿, 方翼. 自动化微生物比浊法测定血清中环丙沙星浓度[J]. 中国临床药理学与治疗学, 1998, 3(3): 190-191.

CHEN Qiang, WANG Rui, FANG Yi. Microbiological turbidimetric method in determination of serum concentrations of ciprofloxaxin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 1998, 3(3): 190-191.

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