慢性肾衰患者低密度脂蛋白亚组份颗粒大小及氧化易感性分析

中国临床药理学与治疗学 ›› 2001, Vol. 6 ›› Issue (3): 248-251.

• 第一届全国临床心脑血管病学术会议论文 • 上一篇下一篇

慢性肾衰患者低密度脂蛋白亚组份颗粒大小及氧化易感性分析

胡兵, 汪俊军², 陈林囡, 庄一义²

南京军区南京总医院中医科;
²全军医学检验中心, 南京 210002

收稿日期:2001-01-10 修回日期:2001-06-25 出版日期:2001-06-26 发布日期:2020-11-30
基金资助:
江苏省应用基础研究课题(BJ 97111)

Analyzing subfraction particle size of low density lipoprotein and its susceptibility to in vitro oxidation in patients with chronic renal failure

HU Bing, WANG Jun-Jun², CHEN Ning-Nan, ZHUANG Yi-Yi²

Department of Traditional Chinese Medicine, Jingling Hospital, Nanjing 210002

Received:2001-01-10 Revised:2001-06-25 Online:2001-06-26 Published:2020-11-30

摘要/Abstract

摘要： 目的研究慢性肾功能衰竭(CRF) 患者低密度脂蛋白(LDL) 亚组份颗粒直径及氧化易感性的异常特征, 探讨其动脉硬化高发生率的机制。方法超速离心分离30 例CRF 患者及26 例对照组的血浆LDL, 采用2%～16%聚丙烯酰胺凝胶梯度电泳法分析LDL 颗粒直径及图形并分析其抗氧化能力。结果 (1) CRF 患者LDL 颗粒直径(26.35±0.59) nm 同对照组(26.41±0.95) nm 间无差别,两组间A 型、B 型LDL 图形分布亦相同;(2) LDL颗粒直径同血浆TG 水平负相关(r=-0.26, P<0.05);(3) CRF 患者LDL 抗氧化能力下降。结论 CRF 患者动脉粥样硬化的高发生率同其LDL 易于氧化的特性相关联。

关键词: 低密度脂蛋白, 慢性肾功能衰竭, 动脉粥样硬化

Abstract: Aim To study the size and susceptibility of low density lipoprotein (LDL) particles in patients with chronic renal failure (CRF).Methods Plasma LDL in 30 CRF patients and 26 control subjects were analyzed by 2%～16% non-denaturing poly acrylamide gradient gels.The continuous monitoring of LDL oxidation by absorbance measurement at 234 nm was performed to analyze the susceptibility of LDL to Cu²⁺ mediated oxidation in CRF patients.Results In LDL particle diameters no dif ference was found between the CRF patients and control subjects[(26.35±0.59) nm vs (26.41±0.95) nm, P>0.05]. The dist ribution of LDL subfraction pat tern also had no difference between two groups.LDL particle diameter was neg atively correlated with plasma t riglycerides (r=-0.26, P<0.05).However, the lagtime in the test for LDL oxidizabilty in CRF patients was signif icantly sho rter than that in the controls [(56.2±23.7) minvs (97.3±41.2) min, P<0.05].Conclusion Circulating LDL in CRF patients was more susceptible to oxidation in vitro than that of controls.This may be associated with increased risk for coronary artery disease.

Key words: LDL, chronic renal failure, atherosclerosis

中图分类号:

R586.12

胡兵, 汪俊军, 陈林囡, 庄一义. 慢性肾衰患者低密度脂蛋白亚组份颗粒大小及氧化易感性分析[J]. 中国临床药理学与治疗学, 2001, 6(3): 248-251.

HU Bing, WANG Jun-Jun, CHEN Ning-Nan, ZHUANG Yi-Yi. Analyzing subfraction particle size of low density lipoprotein and its susceptibility to in vitro oxidation in patients with chronic renal failure[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2001, 6(3): 248-251.

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慢性肾衰患者低密度脂蛋白亚组份颗粒大小及氧化易感性分析

Analyzing subfraction particle size of low density lipoprotein and its susceptibility to in vitro oxidation in patients with chronic renal failure

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