MPP+和6-羟基多巴胺对C6胶质瘤细胞摄取谷氨酸的影响1

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (6): 481-484.

• 研究原著 • 下一篇

MPP⁺和6-羟基多巴胺对C6胶质瘤细胞摄取谷氨酸的影响¹

姚红红, 张芸², 何海蓉, 丁建花, 李皓, 胡刚

南京医科大学药理学与神经生物学系,南京 210029;
²江苏大学医学院,镇江 212001

收稿日期:2002-09-27 修回日期:2002-10-02 出版日期:2002-12-26 发布日期:2020-11-20
通讯作者: 胡刚,男,博士,教授,博士生导师,中国药理学会理事,研究方向为神经退行性疾病的病因学与实验治疗学研究。Tel:025-666-3108 　Fax:025-6528460　Email:ghu@njmu.edu.cn
作者简介:姚红红,女,硕士,讲师。
基金资助:
1 国家自然科学基金项目(No39970846)、教育部优秀青年教师资助计划项目(2001)、教育部高等院校骨干教师资助计划项目(2001)和江苏省卫生厅自然科学基金(NoH9922)资助项目

Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹

YAO Hong-Hong, ZHANG Yun², HE Hai-Rong, DING Jian-Hua, LI Hao, HU Gang

Department of Pharmacology &Neurobiology,Nanjing Medical University,Nanjing 210029;
²Department of Pharmacology,Zhenjiang University,Zhenjiang 212001

Received:2002-09-27 Revised:2002-10-02 Online:2002-12-26 Published:2020-11-20

摘要/Abstract

摘要： 目的研究1-甲基-4-苯基-吡啶离子(MPP⁺)6-羟基多巴胺(6-OHDA)对C6 胶质瘤细胞摄取谷氨酸(Glu)的影响。方法应用放射免疫法测定C6胶质瘤细胞对培养液中[³H] -D,L-谷氨酸的摄取;应用四氮唑(MTT)比色法检测胶质瘤细胞的活性。结果 6-OHDA 和MPP⁺均不同程度地抑制C6 胶质瘤细胞摄取Glu;6-OHDA 显著抑制C6 胶质瘤细胞活性,而MPP⁺却不影响细胞活性;蛋白激酶C(PKC)激动剂TPA 显著增强C6 细胞对Glu 的摄取,并拮抗MPP⁺对C6 细胞摄取Glu 的抑制。结论 6-OHDA 抑制C6 胶质瘤细胞对Glu 的摄取,可能与其抑制细胞活性有关,而MPP⁺的抑制作用可能与直接影响转运体的摄取功能有关,并可能由PKC 途径介导。

关键词: 6-羟基多巴胺, 1-甲基-4-苯基吡啶离子, C6胶质瘤细胞, 谷氨酸, 摄取

Abstract: AIM: To study the effects of 6-OHDA and MPP⁺on the glutamate uptake into cultured C6 glioma cells.METHODS: The glutamate uptake into C6 glioma cells was measured by using radioimmunoassay method;and the viability of C6 glioma cells was investigated by MTT method.RESULTS: Both 6-OHDA and MPP⁺inhibited glutamate uptake into C6 glioma cells in a concentration-dependent manner.Treatment with 6-OHDA produced inhibitory effect on the viability of C6 glioma cells.However,exposure to MPP⁺showed no such effect.Incubation with TPA,a PKC activator,caused increase in glutamate uptake and completely reversed MPP⁺-induced uptake inhibition.CONCLUSION: Inhibition of glutamate uptake by MPP⁺was completely reversed by TPA,indicating that the effect of MPP⁺was mediated by PKC cell signal transduction,while the inhibitory effect of 6-OHDA was due to the impairment viability of C6 glioma cells.

Key words: 6-OHDA, MPP⁺, C6 glioma cells, glutamate uptake

中图分类号:

R971

姚红红, 张芸², 何海蓉, 丁建花, 李皓, 胡刚. MPP⁺和6-羟基多巴胺对C6胶质瘤细胞摄取谷氨酸的影响¹[J]. 中国临床药理学与治疗学, 2002, 7(6): 481-484.

YAO Hong-Hong, ZHANG Yun, HE Hai-Rong, DING Jian-Hua, LI Hao, HU Gang. Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(6): 481-484.

参考文献

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MPP⁺和6-羟基多巴胺对C6胶质瘤细胞摄取谷氨酸的影响¹

Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹

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