人类疱疹病毒7U41的部分功能鉴定与分析

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (6): 485-488.

人类疱疹病毒7U41的部分功能鉴定与分析

张立伐, 唐永煌, 邹平¹, 山西弘一¹

暨南大学医学院第一附属医院感染科,广州 510630;
¹大阪大学医学部微生物科,大阪 565-0871,日本

收稿日期:2002-09-12 修回日期:2002-09-23 出版日期:2002-12-26 发布日期:2020-11-20
通讯作者: 张立伐,男,博士,副教授,研究方向:感染性疾病的分子病毒学研究。Tel/Fax:020-85516832-6138 E-mail:zlif a@yahoo.com

Identification of the function of human herpesvirus 7U41

ZHANG Li-Fa, TANG Yong-Huang, ZOU Ping¹, YAMANISHI Koichi¹

Department of Infectious Diseases,the First Affiliated Hospital of JNUMC,Guangzhou 510630;
¹Department of Microbiology,Osaka University Medical School,C1,Osaka 565-0871,Japan

Received:2002-09-12 Revised:2002-09-23 Online:2002-12-26 Published:2020-11-20

摘要/Abstract

摘要： 目的对人类疱疹病毒7(human herpesvirus 7,HHV-7)U41 的部分功能进行分析鉴定,为药物治疗学探索提供依据。方法免疫荧光检测U41 蛋白的细胞内定位,RT-PCR 检测U41RNA 的表达时相,DNA 结合试验检测U41 蛋白对单、双链DNA 的不同结合能力。结果免疫荧光检测显示,无论在HHV-7 感染细胞或HHV-7 U41 转染细胞,U41 蛋白都是仅在其胞核中弥漫性的分布。在分别加入环己酰亚胺和磷甲酸作为蛋白合成和核酸合成抑制剂感染细胞中,U41 的表达时相检测结果显示U41 蛋白在HHV-7 感染细胞中是一早期表达蛋白。通过DNA结合试验,发现U41 蛋白能结合单链DNA 而不能结合双链DNA 。结论 U41 的主要功能是保持DNA 模板的合适构型,以便DNA 的延伸和病毒DNA 的合成。

关键词: 人类疱疹病毒, 开放读框, 免疫荧光, 环己酰亚胺

Abstract: AIM: To identify the function ORF(open reading frame)U41 of human herpesvirus 7(HHV-7).METHODS: Immunofluorescent detection was used to show the localization of the U41 protein in cells.The expression kinetics of the U41 gene in the presence of cycloheximide(CHX)and phosphomoformic acid(PFA),which are inhibitors of protein synthesis and of viral DNA synthesis respectively,was detected with RT-PCR.DNA binding assay was used to detect the single and double DNA strands binding ability of U41 protein.RESULTS: The U41 protein was expressed as an early protein in the HHV-7 infected cells and localized only in the nuclei of infected cells.The U41 protein was able to bind to a single-strand DNA cellulose column but not to double-strand DNA cellulose column from DNA binding assay.CONCLUSION: The major function of U41 may be to keepthe DNA template in the optimal conformation for DNA elongation and for viral DNA synthesis.

Key words: herpesvirus, open reading frame, immunofluorescence, cycloheximide, phosphomoformic acid

中图分类号:

R511
R752.1

张立伐, 唐永煌, 邹平¹, 山西弘一¹. 人类疱疹病毒7U41的部分功能鉴定与分析[J]. 中国临床药理学与治疗学, 2002, 7(6): 485-488.

ZHANG Li-Fa, TANG Yong-Huang, ZOU Ping, YAMANISHI Koichi. Identification of the function of human herpesvirus 7U41[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(6): 485-488.