关键词: 石杉碱甲透皮控释制剂(贴片), 督脉, 穴位, 一般部位, 记忆障碍, 胆碱酯酶

Abstract: AIM: To observe the improvement function of huperzine-A transdermal drug delivery system(Hup-A TDDS)administration at Governor Vessel(GV)acu-points on memory disorders induced by scopolamine in mice and to compare the effect of GV acu-points administration with general place administration.METHODS: Stepdown test and “Y” maze test were used to observe the influence of Hup-A TDDS administration at GV acu-points on memory disorders induced by scopolamine.Some biochemical indices were also etermined.RESULTS: (1)The marked effects were found among 3 -6 h after Hup-A TDDS administration at GV baihui acupoint.The best effects showed 4 h after administration,and it weakened a little from 5 h to 6 h in 3.4 μg/0.25cm² group.At 26.7 μg/0.25 cm² group,the drug action maintained an effective level from 3 h to 6 h,and reached the maximum at 4 h.(2)Among 5 acu-points of GV to administration at Hup-A TDDS 26.7μg/0.25cm²,the obvious effects on memory disorders existed,and baihui(GV20)was the most effective acu-point.(3)The administration of Hup-A TDDS(26.7μg/0.25cm² and13.4 μg/0.25cm²)through both acu-point and general place(buttocks),approved memory improvement function by stepdown test and “Y” maze test,and the action in baihui acu-point groupshowed more significant.The depressed trend of cholinesterase activity in brain was proved in all of both GV acu-point and general place groups and baihui acr-point groupexposed signal difference when compared with mokcl group.CONCLUSION: Hup-A TDDS administration at GV acu-point shows a better improvement of memory disorders in comparison with general place.The action mechanism may be due to the depressed cholinesterase activity and the improved cholinergic system function.

Key words: huperzine-A transdermal drug delivery system(Hup-A TDDS), Governor Vessel, acu-point, general place, memory disorders, step down test, "Y"maze test, cholinesterase