关键词: 脑缺血, 果糖二磷酸钠镁, 1, 6-二磷酸果糖, 硫酸镁, 突触体, 乳酸, Na⁺-K⁺ATP 酶, Ca^{2 +}-Mg²⁺ATP 酶

Abstract: AIM: To study the effects of sodium magnesium fructose diphosphate(FDPM)on lactate concentration and ATPase activity of ischemic synaptosome,so as to explore the protective mechanisms of FDPM on cerebral ischemia.METHODS: Synaptosome prepared from normal rat brain was cultured with oxygen and glucose deprived to establish the model of ischemic synaptosome.1.3 mmol·L^-1magnesium sulfate,4.0mmol·L^-1 fructose-1,6-diphosphate(FDP)and 1.3 mmol ·L^-1 FDPM were incubated with the ischemic synaptosomes separately.After 60min,the lactate concentration as well as the activities of Na⁺-K⁺ATPase and Ca²⁺-Mg²⁺ATPase were detected.RESULTS: FDPM reduced the lactate concentration,raised the activities of Na⁺-K⁺ATPase and Ca²⁺-Mg²⁺ATPase of ischemic synaptosomes(p<0.05).The effects of FDPM were stronger than those of FDp(p<0.05).CONCLUSION: FDPM may protect cerebral ischemia by improving the energy metabolism,and mitigating acidosis state of brain tissue.

Key words: cerebral ischemia, sodium magnesium fructose diphosphate, fructose-1, 6-diphosphate, magnesium sulfate, synaptosome, lactate, Na⁺-K⁺ATPase, Ca²⁺-Mg²⁺ATPase