欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (2): 170-172.

• 研究原著 • 上一篇    下一篇

KATP通道介导的油茶皂甙对在体大鼠心肌产生的缺血预适应样保护作用1

黄起壬, 曹守仪, 何明, 李萍, 彭维杰   

  1. 江西医学院药理教研室, 南昌 330006, 江西
  • 收稿日期:2002-10-14 修回日期:2002-11-11 出版日期:2003-04-26 发布日期:2020-11-25
  • 通讯作者: 黄起壬, 男, 在读博士, 讲师, 研究方向:心肌保护。Tel:0791-8603244 E-mail:huangqr@jxmu.edu.cn
  • 基金资助:
    1 江西省自然科学基金资助项目(№C950102)

Protective effects as ischemic preconditioning of sasanquasaponin mediated by KATP channel in the intact rat hearts1

HUANG Qi-Ren, Cao Shou-Yi, HE Ming, LI Ping, PENG Wei-Jie   

  1. Department of Pharmacology, Jiangxi Medical College, Nanchang 330006, Jiangxi
  • Received:2002-10-14 Revised:2002-11-11 Online:2003-04-26 Published:2020-11-25

摘要: 目的: 研究油茶皂甙(SQS) 对大鼠心肌产生的缺血预适应样保护作用及与KATP 通道的关系。方法: 以ISO 诱发大鼠心肌缺血损伤为模型, 使用KATP通道特异性阻断剂gliberclamide (GLI 5 mg·kg-1)。实验分为四组, 分别为生理盐水对照组(NS 组)、ISO诱发损伤组(I/R 组)、SQS 组(I/R +SQS 0.2mg·kg-1) 和GLI 组(I/R +GLI +SQS)。在注射ISO前, 从阴茎静脉预适应注射各被试药物或NS, 每天1次, 连续3 d。末次给药后立即皮下多点注射ISO。NS 组皮下注射等量NS。分别测定大鼠ECG 及末次给药后血清CPK 活性, FFA 和腺苷含量。结果: 预适应iv SQS 能有效地保护ISO 所致大鼠心肌损伤;先iv GLI 后再给予SQS, 则SQS 对心肌损伤的保护作用明显减弱。结论: SQS 对ISO 所致心肌缺血大鼠可产生药理性预适应保护作用, 该作用可能由KATP通道所介导。

关键词: 药理学, 油茶皂甙, KATP 通道, 心肌缺血预适应, 心肌保护

Abstract: AIM: To study protective effects as myocardial ischemic preconditioning of sasanquasaponin (SQS) and its relationship with KATP channel. METHODS: The study adopted the model of myocardial ischemic injury induced by subcutaneous injection of isoproterenol (ISO) in rats, administering specific KATP channel blocker gliberclamide (GLI 5 mg·kg -1 ).Four groups were set as NS group, I/R group, SQS group (0.2 mg·kg -1), and GLI group (5 mg·kg -1).Prior to injection of ISO, all agents were intraveneously injected into rats for 3 days, one time per day.Subsequently, ISO was subcutaneuously injected into rats by the ways of many different sites, and some indices were measured including ECG, serum creatine kinase (CK) activity, free fatty acid (FFA), and adenosine contents in rats. RESULTS: Preconditioningly intravenous injection of SQS could effectively protect myocardium from ischemic injury induced by ISO.With GLI injected prior to SQS, the cardioprotective effects of SQS were significantly attenuated. CONCLUSION: SQS can protect myocardium from ischemic injury induced by ISO, and the protection may be mediated by KATP channel.

Key words: pharmacology, sasanquanonion, KATP channel, myocardial ischemic preconditioning, cardioprotection

中图分类号: