灵芝多糖对大鼠胰岛细胞分泌胰岛素功能的影响

中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (3): 265-268.

灵芝多糖对大鼠胰岛细胞分泌胰岛素功能的影响

张慧娜, 林志彬

北京大学基础医学院药理学系, 北京 100083

收稿日期:2003-02-18 修回日期:2003-02-24 出版日期:2003-06-26 发布日期:2020-11-25
通讯作者: 林志彬, 男, 教授, 博士生导师, 中国药理学会理事长, 研究方向为内分泌和免疫药理学。Tel:010-62091686 (Fax) E-mail:linzb@public3.bta.net.cn
作者简介:张慧娜, 女, 博士生。
基金资助:
上海绿谷(集团) 有限公司科研基金资助课题

Effect of Ganoderma lucidum polysaccharides on the insulin releasing function of pancreatic islets in rats

ZHANG Hui-Na, LIN Zhi-Bin

Department of Pharmacology, Basic Medical College of Peking University, Beijing 100083

Received:2003-02-18 Revised:2003-02-24 Online:2003-06-26 Published:2020-11-25

摘要/Abstract

摘要： 目的: 探讨灵芝多糖(Gl-PS) 对胰岛细胞分泌胰岛素的影响。方法: 分离大鼠胰岛细胞, 分别观察在葡萄糖为5.6 和16.7 mmol·L^-1时, Gl-PS 对胰岛细胞分泌胰岛素的影响。进一步加入不同的抑制剂观察Gl-PS 对胰岛细胞分泌胰岛素的影响。采用Western blotting 观察Gl-PS 对胰岛细胞葡萄糖转运蛋白2(GLUT2) 表达的影响。结果: 在5.6 mmol·L^-1葡萄糖时, 100 mg·L^-1的Gl-PS 可明显促进胰岛细胞胰岛素的分泌;当葡萄糖浓度为16.7 mmol·L^-1时,50 和100 mg·L^-1的Gl-PS 均可明显促进胰岛细胞胰岛素的分泌。维拉帕米或维拉帕米+EGTA 均可显著抑制5.6 和16.7 mmol·L^-1 葡萄糖时胰岛素的分泌, 维拉帕米只能部分抑制Gl-PS 的促胰岛素分泌作用, 而维拉帕米+EGTA 则可完全抑制Gl-PS 的这种作用。在葡萄糖浓度为5.6 和16.7mmol·L^-1时,Gl-PS 50 和100 mg ·L^-1 均能明显促进胰岛细胞GLUT2 的蛋白表达。结论: Gl-PS 可能通过促进胰岛细胞GLUT2 蛋白的表达从而有助于葡萄糖转运入B细胞, 促进葡萄糖的代谢, 引起胰岛细胞外Ca^{2 +}内流而起到促胰岛素释放的作用。

关键词: 药理学, 灵芝多糖, 胰岛细胞, 胰岛素分泌, 葡萄糖转运蛋白2

Abstract: AIM: To investigate the effect of (Ganoderma lucidum) polysaccharides (Gl-PS) on the insulinreleasing function of pancreatic islets. METHODS: Pancreatic islets were isolated and incubated with 5.6 or 16.7 mmol·L ^-1 glucose and different concentrations of Gl-PS for 1 h.Then the insulin was examined.Verapamil, and verapamil combined EGTA were used to testify whether the insulin-releasing effect of Gl-PS was inhibited by these inhibitors.The effects of Gl-PS on protein expression of the glucose transporter 2(GLUT2) under 5.6 and 16.7 mmol·L^-1 glucose were also investigated. RESULTS: Gl-PS stimulated the insulin release when incubated with the glucose of 5.6 or 16.7mmol·L^-1.The insulin releasing effect of Gl-PS was partly inhibited by verapamil and completely inhibited by verapamil +EGTA. Gl-PS could promote the GLUT2 protein expression of pancreatic islets under glucose of 5.6 and 16.7 mmol·L ^-1. CONCLUSION: Gl-PS possesses insulinreleasing effect under the glucose of 5.6 and 16.7 mmol·L ^-1 due to the promotion of the GLUT2 protein expression and the subsequent facilitation of Ca^{2 +} inflow into the pancreatic B cells.

Key words: pharmacology, Ganoderma lucidum polysaccharides, pancreatic islets, insulin-releasing function, glucose transporter 2

中图分类号:

张慧娜, 林志彬. 灵芝多糖对大鼠胰岛细胞分泌胰岛素功能的影响[J]. 中国临床药理学与治疗学, 2003, 8(3): 265-268.

ZHANG Hui-Na, LIN Zhi-Bin. Effect of Ganoderma lucidum polysaccharides on the insulin releasing function of pancreatic islets in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(3): 265-268.

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