白介素-1受体拮抗剂对致敏豚鼠的抗喘作用

中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (5): 506-510.

白介素-1受体拮抗剂对致敏豚鼠的抗喘作用

张洪泉, 许正新, 葛晓群, 李吉平, 孙云, 柳丽

扬州大学医药研究所,扬州 225001,江苏

收稿日期:2003-04-30 接受日期:2003-05-08 出版日期:2003-10-26 发布日期:2020-11-19

Anti-asthmatic effects of interleukin-1 receptor antagonist in sensitized guineaPigs

ZHANG Hong-Quan, XU Zheng-Xin, GE Xiao-Qun, LI Ji-Ping, SUN Yun,LIU LI

Medical,Pharmaceutical Academe of Yangzhou University,Yangzhou225001,Jiangsu,China

Received:2003-04-30 Accepted:2003-05-08 Online:2003-10-26 Published:2020-11-19
Contact: ZHANG Hong-Quan,male,director,professor,engaged in pulmonary pharmacology.Tel:0514-7978877 　Fax:0514-7978821
Supported by:
Project supported by 863 National Natural S cience Foundation of China(№2001AA215251)

摘要/Abstract

摘要： 目的观察白介素-1 受体拮抗剂(IL-1ra)对致敏豚鼠的抗喘作用。方法用卵白蛋白致敏豚鼠,用张力换能器和MedLab 软件记录气道平滑肌的肌张力和收缩活动。结果整体实验时,在0.33 ~9.0 mg·kg^-1剂量范围内,IL-1ra 能使组胺-乙酰胆碱及卵白蛋白诱发的致敏豚鼠哮喘的潜伏期延长,动物跌倒率降低。此作用具有剂量依赖性。其中,9.0 mg·kg-1剂量组的作用与异丙基肾上腺素相同;离体实验时,3×10^-7mol·L^-1以上浓度的IL-1ra 对气管平滑肌具有直接松弛作用,其EC50 为5.88×10^-7mol·L^-1;10-7 mol·L^-1的IL-1ra 能对抗卵白蛋白引起的气管平滑肌肌张力的增加;3×10^-7mol·L^-1以上剂量组的IL-1ra 预防给药则能使卵白蛋白诱发的气管平滑肌的收缩程度显著降低,其IC₅₀为4.48×10^-7mol·L^-1。结论 IL-1ra 能直接松弛致敏豚鼠的气管平滑肌,拮抗多种抗原诱发的致敏豚鼠的哮喘发作。

关键词: 药理学, 白介素-1, 受体拮抗剂, 豚鼠, 组胺, 乙酰胆碱, 卵白蛋白, 哮喘

Abstract: AIM: To observe the anti-asthmatic effects of interleukin-1 receptor antagonist (IL-1ra)in sensitized guineaPigs.METHODS: Sensitized guineaPigs were made by ovalbumin,and the tension and contraction of tracheal smooth muscles were measured by force-displacement transducer and MedLab software.RESULTS: In the doses of 0.33 -9.0 mg·kg^-1,IL-1raProlonged the asthmatic incubationPeriod and reduced animal tumble ratio in asthma guineaPigs induced by histamine-acetylcholine or ovalbumin.These effects were dose-dependent.In vitro,IL-1ra could directly relax tracheal smooth muscles uPthe dose of 3×; 10^-7mol·L^-1and the EC50 value was 5.88×; 10^-7mol·L^-1.IL-1ra antagonized the increase of trachea tension stimulated by ovalbumin at the dose of 10^-7mol·L^-1.Moreover,IL-1raPrevented tracheal smooth muscle contraction induced by ovalbumin uPthe dose of 3×; 10^-7mol·L^-1,and the IC₅₀value was 4.48×; 10^-7mol·L^-1.CONCLUSIONS: IL-1ra can directly relax tracheal smooth muscles in sensitized guineaPigs,and antagonize asthma caused by many kinds of antigens.;

Key words: pharmacology, interleukin-1, receptor antagonist, histamine, acetylcholine, asthma, contraction, guineaPigs

中图分类号:

R965.1

张洪泉, 许正新, 葛晓群, 李吉平, 孙云, 柳丽. 白介素-1受体拮抗剂对致敏豚鼠的抗喘作用[J]. 中国临床药理学与治疗学, 2003, 8(5): 506-510.

ZHANG Hong-Quan, XU Zheng-Xin, GE Xiao-Qun, LI Ji-Ping, SUN Yun, LIU LI. Anti-asthmatic effects of interleukin-1 receptor antagonist in sensitized guineaPigs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(5): 506-510.

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