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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (5): 541-543.

• 研究原著 • 上一篇    下一篇

小牛血去蛋白注射液对大鼠脑缺血再灌注损伤的保护作用

赵明, 王洪新, 王桂君, 李淑媛1   

  1. 锦州医学院药理教研室,锦州 121000,辽宁;
    1连医科大学药理教研室,大连 116027,辽宁
  • 收稿日期:2003-04-28 修回日期:2003-05-13 出版日期:2003-10-26 发布日期:2020-11-19
  • 通讯作者: 王洪新,男,医学博士,教授,硕士生导师,研究方向:心血管药理。Tel:0416-4580209  E-mail:jyhxwang@hotmail.com
  • 作者简介:赵明,女,药理学硕士,主治医师。

Protection of deproteinised calf blood injection on cerebral ischemia and reperfusion injury in rats

ZHAO Ming, WANG Hong-Xin, WANG Gui-Jun, LI Shu-Yuan1   

  1. Department ofPharmacology,Jinzhou Medical College,Jinzhou121001,liaoning,China;
    1Department ofPharmacol-ogy,Dalian Medical University,Dalian 116027,liaoning,China
  • Received:2003-04-28 Revised:2003-05-13 Online:2003-10-26 Published:2020-11-19

摘要: 目的 研究小牛血去蛋白注射液(deproteinized calf blood injection,DCBI)对大鼠脑缺血及再灌注损伤的保护作用 。方法 采用 SD 大鼠双侧颈总动脉结扎法制作不完全性脑缺血再灌注模型,观察 DCBI对脑缺血再灌注 SD 大鼠的脑组织水含量,脑组织丙二醛(MDA)含量的影响,并通过光镜观察其病理组织学变化 。结果 缺血组与缺血再灌注组脑组织水含量 、MDA 含量较假手术组明显增高;DCBI 组脑组织水含量、MDA 含量较缺血组与缺血再灌注组下降。病理组织学检查可见缺血组与假手术组相比大脑皮层细胞出现肿胀改变 。缺血再灌注组脑组织水肿加剧,细胞周围呈海绵状 。用药组脑细胞仅有轻度肿胀改变。结论 DCBI 对大鼠脑缺血及缺血再灌注损伤具有保护作用 。

关键词: 药理学, 脑缺血, 再灌注损伤, 牛血去蛋白注射液, 自由基, 脂质过氧化作用

Abstract: AIM: To investigate theProtection of the deproteinised calf blood injection (DCBI)against cerebral ischemia-reperfusion injury in the Sprague-Dawley rats.METHODS: The occlusion of bilateral carotid arteries in rats was used to make the cerebral ischemia-reperfusion models.After 30 min ischemia and 20 min reperfusion,bilateral cortex was taken out to measure the content of water and MDA,respectively.Moreover,immunofluores-cent stain method in cortex tissue slice was used.RE-SULTS: The contents of water andMDAmarkedly elevat-ed in cortex 1 h after ischemia-reperfusion.DCBI at 30 or 60 mg·kg-1dosesPrior to ischemia couldPartly reverse the elevation of the water and MDA contents.Morphologi-cal investigation bylight microscope showed the cell tu-mescent in ischemia grouPcompared with sham operation group.Reperfusion following ischemialeaded to aggrava-tion of the cell dropsy and the surroundingPresented spongy alteration;whereas the cells in DCBI-treated groupswere only slightly swelled.CONCLUSION: DC-BIProvides significantProtective effect against cerebral ischemia-repefusion injury.

Key words: pharmacology, cerebral ischemia, cere-bral ischemia-reperfusion injury, deproteinised calf blood injection, oxygen free radicals, lipidPeroxidation

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