吴茱萸次碱对小鼠溃疡性肠炎的治疗作用

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (11): 1273-1277.

吴茱萸次碱对小鼠溃疡性肠炎的治疗作用

刘保林, 吴巍巍, 靳长峰, 王音

中国药科大学中药药理教研室,南京 210038,江苏

收稿日期:2004-07-27 修回日期:2004-10-25 出版日期:2004-11-26 发布日期:2020-11-19
通讯作者: 刘保林,副教授,硕士生导师,研究方向:中药药理和免疫药理Tel: 025-8532209 E-mail: njlbl55@sohu.com

Effects of rutaecarpine on ulcerative colitis in mice

LIU Bao-Lin, WU Wei-Wei, JIN Chang-Feng, WANG Yin

Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, Jiangsu,China

Received:2004-07-27 Revised:2004-10-25 Online:2004-11-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 观察吴茱萸次碱治疗小鼠急性结肠炎的药效及对豚鼠肠道平滑肌运动的影响。方法: 2,4-二硝基氯苯(DNCB)/乙醇灌肠制作小鼠急性结肠炎模型,经口给予吴茱萸次碱10、30、100 mg·kg^-1,观察吴茱萸次碱对小鼠腹泻,组织形态损伤,结肠组织PGE₂含量、MPO活力以及对乙酰胆碱、组胺作用下豚鼠离体肠管收缩运动的影响。结果: DNCB诱发结肠炎后,小鼠出现腹泻、充血、溃疡、肠壁增厚等变化。经口给予吴茱萸次碱后,可以显著改善小鼠的腹泻、组织损伤,并且能够降低结肠组织中MPO活性和PGE₂的含量,对乙酰胆碱及组胺作用下豚鼠离体肠管收缩亦表现出显著的对抗作用。结论: 吴茱萸次碱可以抑制炎性浸润、渗出和组织增生,减轻结肠黏膜的病理损害和抑制肠道平滑肌运动,对实验性动物急性结肠炎有较好的治疗作用。

关键词: 吴茱萸次碱, 溃疡性结肠炎, 髓过氧化物酶(MPO), PGE₂, 肠管收缩

Abstract: AIM: To evaluate the effects of rutae-carpine on ulcerative colitis and intestinal movement in experimental animals. METHODS: The mouse model of acute colitis was induced by colonic instillation of dinitro-chlorobenzene (DNCB)/alcohol. Rutaecarpine (10, 30, and 100 mg·kg^-1) were orally administrated to animals for 3 days. Diarrheas, colon weight index, macroscopic damage in the colon were observed and the activity of MPO and the content of PGE₂ in colon were also exam-ined.Meanwhile, the inhibitory effects of rutaecarpine on ileal contraction movement in Guinea pig which was in-duced by acetylcoline or histamine in vitro were exam-ined. RESULTS: The diarrhea and the colon weight in-dex were reduced and the macroscopic damage in the in-flammatory colon was effectively attenuated by mtae-carpine. The elevated level of MPO and PGE₂ were also significantly decreased. Ileal contraction movement in-duced by acetylcoline or histamine in vitro was strongly dose-dependent inhibited by rutaecarpine. CONCLUSION: Rutaecarpine can effectively inhibit the inflamma-tory infiltration, bleeding and proliferation in experimental ulcerative colitis and the movement of intestinal smooth muscle. Rutaecarpine may be an effective drug in treating inflammatory bowel diseases in clinic.

Key words: rutaecarpine, ulcerative colitis, my-eloperoxidase (MPO), prostaglandin K (PGE₂), intestinal contraction

中图分类号:

R965

刘保林, 吴巍巍, 靳长峰, 王音. 吴茱萸次碱对小鼠溃疡性肠炎的治疗作用[J]. 中国临床药理学与治疗学, 2004, 9(11): 1273-1277.

LIU Bao-Lin, WU Wei-Wei, JIN Chang-Feng, WANG Yin. Effects of rutaecarpine on ulcerative colitis in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(11): 1273-1277.

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