欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (11): 1278-1280.

• 研究原著 • 上一篇    下一篇

前列腺素E1对大鼠移植肺再灌注损伤的保护作用

周勇安, 刘锟, 张涛, 谷仲平   

  1. 第四军医大学唐都医院胸外科,西安 710038,陕西
  • 收稿日期:2004-07-20 修回日期:2004-10-10 出版日期:2004-11-26 发布日期:2020-11-19
  • 作者简介:周勇安,男,博士生,从事肺和食管外科临床研究。Tel: 029-83377736 E-mail: zhouyafnimu@yahoo.com.cn; 刘锟,男,教授,博士生导师,从事肺和食管外科临床研究。Tel: 029-83377736 E-mail: liukun@fmmu.edu.cn

Protective effects of prostaglandin E1 on ischemia/reperfusion-induced lung injury after lung transplantation in rats

ZHOU Yong-An, LIU Kun, ZHANG Tao, GU Zhong-Ping   

  1. Department of Thoracic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, Shaanxi, China
  • Received:2004-07-20 Revised:2004-10-10 Online:2004-11-26 Published:2020-11-19

摘要: 目的: 探讨前列腺素E1(PGE1)对大鼠移植肺再灌注损伤的保护作用及机制。方法: SD大鼠36只随机分为3组,每组12只,即对照组、肺移植组和肺移植加PGE,处理组,观察受体大鼠肺移植前后注射PGE1,对移植肺再灌注损伤的保护作用。肺功能指标包括肺湿干重比、肺通透性指数、支气管肺泡灌洗液(BALF)中白细胞计数和分类。比色法检测各组肺组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量。ELISA法检测受体大鼠血清中肿瘤坏死因子α(TNFα)含量。结果: 肺移植术后1 h,肺湿干重比、肺通透指数、BALF中中性粒细胞百分比和MDA含量显著高于对照组< 0.01),肺组织中SOD活性明显低于对照组(P< 0.01);给予受体大鼠静脉注射PGE1可明显改善上述指标(P <0.01);肺移植组血清TNFα含量较对照组显著升高(P < 0.01), PGE1可明显降低血清TNFα水平(P<0.01)。结论: PGE1对移植肺缺血/再灌注损伤有显著的保护作用,与其抗氧化自由基损伤、抑制中性白细胞激活和炎性因子TNFα分泌有关。

关键词: 肺移植, 缺血/再灌注, 前列腺素E1, 胖瘤坏死因子α

Abstract: AIM: To investigate the protective effects and the mechanisms of prostaglandin E1(PGE1) on isch-emia/reperfusion (I/R)-induced lung injury after lung transplantation in rats. METHODS: 36 SD rats were randomly divided into 3 groups (n=12 in each): sham operation group (control group), lung transplantation (LT)group and PGE1, treatment group. PGEi was admin-istered to the rats through intra-venous way from 10 min before the operation to the end of the reperfusion. The wet/dry ratio of lung, lung permeability index and neutro-phil percentage were detected in bronchoalveolar lavage fluid (BALF). Superoxide dismutase (SOD) and malond-ialdehyde (MDA) of lung tissue were measured by color-imetry.Serum level of tumor necrosis factor a(TNFα) was detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: The wet/dry ratio of lung, lung permeability index and neutrophils percentage in BALF and MDA content of lung tissue in LT group were higher than those in control group(P < 0.01). SOD activity of lung tissue in LT group was lower than that in control group(P < 0.01). Administration of PGE1, improved the indexes above (P < 0.01). Serum level of TNFα in-creased after lung transplantation compared with the con-trol group. However, PGE1 treatment reduced the serum levelof TNFα(P<0.01). CONCLUSION: PGE1 can protect the lung function against I/R injury after lung transplantation. The mechanism is related to its anti-oxi-dative effect and the inhibition of TNFα release and neu-trophils activation.

Key words: lung transplantation, ischemia-reperfu-sion, prostaglandin E1, tumor necrosis factor a

中图分类号: