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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (11): 1289-1292.

• 研究原著 • 上一篇    下一篇

桂枝汤有效部位A对IL-1剌激的大鼠脑微血管内皮细胞前列腺素E2及其主要信号转导元件的影响

姜楠, 霍海如, 李兰芳, 郭淑英, 姜廷良   

  1. 中国中医研究院中药研究所唐氏中药研究中心,北京 100700
  • 收稿日期:2004-08-23 修回日期:2004-09-23 出版日期:2004-11-26 发布日期:2020-11-19
  • 通讯作者: 霍海如,女,博士,研究方向:中药对细胞信号转导的影响 Tel: 010-64041008 E-mail: huohr@yahoo.com.cn
  • 作者简介:姜楠,女,硕士研究生,研究方向:中药对细胞信号转导的影响。
  • 基金资助:
    国家自然科学基金重大研究计划资助项目(No90209006)

Effects of Fr. A in Guizhi Tang on the excretion of PGE2 and its signal transduction components in cerebral microvascular endothelial cells stimulated by IL-1

JIANG Nan, HUO Hai-Ru, Li Lan-Fang, GUO Shu-Ying, JIANG Ting-Liang   

  1. Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100700, China
  • Received:2004-08-23 Revised:2004-09-23 Online:2004-11-26 Published:2020-11-19

摘要: 目的: 探讨桂枝汤解热有效部位A(Fr.A)对IL-1刺激的大鼠脑微血管内皮细胞(rCMEC)PGE2信号转导通路主要元件的影响。方法: 制备大鼠含药血清,通过放射免疫法测定PGE2含量,酶反应底物法测定PLA2活性,ELISA法测定C0X活性,比色法测定NO含量。结果: Fr. A含药血清处理iCMEC后,在IL-1刺激下,孵育液中PQE2和NO含量、总C0X和C0X-2活性均显著减低,对升高的SPLA2活性无明显影响。结论: Fi.A可通过影响PGE2信号转导通路主要元件C0X及N0,进而影响PGE2水平。

关键词: 桂枝汤有效部位A, 脑微血管内皮细胞, 白细胞介素-1, 前列腺素, 信号转导

Abstract: AIM: To investigate the effects of Fr. A, an antipyretic active fraction extracted from Guizhi Tang, on the excretion of PGE2 and its signal transduction com-ponents in cerebral microvascular endothelial cells (rCMEC) stimulated by IL-1. METHODS: Fr. A-containing rat serums were prepared. The PGE2, PLA2, COX and NO were measured by radioimmunoassay, enzyme reaction substrate assay, ELISA and colorimetry, respectively. RESULTS: When administration of Fr. A-containing serum, contents of PGEj and NO, the activity total COX and of COX-2 were significantly decreased in rCMEC. But the activity of sPLA2 was not significantly decreased. CONCLUSION: Fr. A can affect the level of PGE2 by iniluencing the main elements of COX and NO in PGE2 signal transduction passway.

Key words: active fraction A of Guizhi Tang, cere-bral microvascular endothelial cells, interleukin-1, pros-taglandin E2, signal transduction

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