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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (11): 1293-1296.

• 研究原著 • 上一篇    下一篇

小剂量茶碱对原始T淋巴细胞分化的影响

聂汉祥, 郭卫, 杨炯   

  1. 武汉大学人民医院呼吸内科,武汉 430060,湖北
  • 收稿日期:2004-07-07 修回日期:2004-11-09 出版日期:2004-11-26 发布日期:2020-11-19
  • 通讯作者: 聂汉祥,男,硕士,讲师,研究方向:哮喘的发病机制和治疗。Tel: 027-88041919-2215 E-mail: WLSCC@public.wh.hb.cn
  • 作者简介:杨炯,男,医学博士,教授,博士生导师,研究方向:哮喘的发病机制和治疗。
  • 基金资助:
    湖北省教育厅科研计划(No2001A14015)

Effects of low-dose theophylline on differentiation of naive T lymphocyte

NIE Han-Xiang, GUO Wei, YANG Jiong   

  1. Department of Respiratory Disease, Wuhan University Remin Hospital, Wuhan 430060, Hubei, China
  • Received:2004-07-07 Revised:2004-11-09 Online:2004-11-26 Published:2020-11-19

摘要: 目的: 研究小剂量茶碱对原始T淋巴细胞分化的影响。方法: 分离新生儿脐血原始T淋巴细胞和单核细跑,将茶碱(10 mg·L-1)加入植物血凝素(PHA,200 mg·L-1)刺激下的新生儿脐带血原始T淋巴细胞,与单核细胞共培养3 d,流式细胞仪检测原始T淋巴细胞分化为Thl和Th2的水平,酶联免疫吸附法(ELISA)检测培养上清液中白介素-4(IL-4)、白介素-5(IL-5)和干扰素-7(IFN-7)浓度。结果: 茶碱显著促进原始T淋巴细胞向Thl细胞分化(P<0.05),但对Th2细胞分化没有显著影响,Thl/Th2比例增加(P <0.05);茶碱可使IL-4和IL>5的生成量显著降低,1例-7的生成量显著增加(P<0.05)。结论: 茶碱可调节原始T淋巴细胞分化并可能由此而发挥其抗炎作用。

关键词: 哮喘, 茶碱, Thl细胞, Th2细胞, 细胞因子

Abstract: AIM: To investigate the effects of low-dose theophylline on differentiation of the naive T lympho-cyte.METHODS: Naive T lymphocytes and monocytes were isolated from cord blood in neonate. Theophylline (10 mg·L-1) was added in naive T lymphocytes and monocytes which stimulated by PHA (200 mg·L-1) for 3 days, and the differentiation of T cells was analyzed by flow cytometry. Cytokines were measured by enzyme linked immuno-sorbent assay (ELISA). RESULTS: Theophylline significantly promoted differentiation from Thl cells to naive T cells (P < 0.05), but it had no sig-nificant effect on differentiation to Th2. The ratio of Thl/Th2 was increased (P < 0.05). The production of IL-4 and IL-5 were lower and IFN-y was higher than that in the control group (P < 0.05). CONCLUSION: Low-dose theophylline can modulate the differentiation of naive T cells and exert its anti-inflammatory effect.

Key words: phaimacodynainics, asthma, theophylline, Thl cells, Th2 cells, cytokine

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