C2-神经酰胺诱导大鼠脑胶质瘤细胞C6 的早期凋亡作用

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (12): 1349-1352.

C2-神经酰胺诱导大鼠脑胶质瘤细胞C6 的早期凋亡作用

祁红, 刘艳梅, 吴兴军, 史桂英¹, 陈红专

上海第二医科大学药理学教研室, ¹细胞生物学教研室, 上海 200025

收稿日期:2004-10-13 修回日期:2004-11-23 出版日期:2004-12-26 发布日期:2020-11-19
通讯作者: 祁红, 女, 博士, 副教授, 研究方向:肿瘤药理学。Tel:021-63846570-776450 E-mail:qihoo2000@yahoo.com.cn
基金资助:
上海市教委重点学科基金资助(No2003JY04)

Induction effects of C2-ceramide on early apoptosis in rat glioma cell C6

QI Hong, LIU Yang-Mei, WU Xing-Jun, SHI Gui-Ying¹, CHEN Hong-Zhuan

Department of Pharmacology, ¹Department of Cell Biology, Shanghai Second Medical University, Shanghai 200025, China

Received:2004-10-13 Revised:2004-11-23 Online:2004-12-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 研究外源性C2-神经酰胺对大鼠脑胶质瘤细胞C6活力的抑制作用以及早期凋亡诱导作用。方法: 采用MTT 法测定C2-神经酰胺对C6 细胞活力的影响;倒置荧光显微系统观察细胞形态变化;AnnexinⅤ PI 双染色流式细胞术对细胞早期凋亡进行定量分析。结果: C2-神经酰胺可显著抑制C6 细胞的活力, IC₅₀为2.2×10^-5 mol·L^-1;荧光染色可见核浓染等细胞凋亡特征形态改变;流式细胞术分析表明C2-神经酰胺可诱导C6 细胞发生早期凋亡作用,且凋亡百分率呈时间及浓度依赖性, C2-神经酰胺2×10^-5 mol·L^-1处理24 h 后平均早期凋亡率高达49.3 %。结论: C2-神经酰胺主要通过诱导早期细胞凋亡对大鼠脑胶质瘤C6 细胞发挥细胞毒作用, 提高神经酰胺水平有望成为肿瘤化疗的新途径。

关键词: C2-神经酰胺, C6, Annexin Ⅴ/PI, 早期凋亡

Abstract: AIM: To investigate the effects of exogenous cell-permeable C2-ceramide on viability and early apoptosis induction in rat glioma cell C6.METHODS: The effects of C2-ceramide on viability of C6 cell after 24 h treatment were assessed by MTT test.The cellular morphology was observed by Olympus inverted reflected light fluorescence observation attachment.The early events of the apoptosis were recorded by flow cytometry analysis with a combination of Annexin V-FITC and propium iodide (PI) which was used for staining cells.RESULTS: C2-ceramide significantly inhibited the cell viability of C6 cells and the IC₅₀ was 2.2×10^-5mol·L^-1.The classical apoptotic morphology change was induced by C2-ceramide, and the early cell apoptosis was induced in a time and concentration-dependent manner.After treated with 2×10^-5mol·L^-1C2-ceramide for 24 h, the average apoptotic death rate of cells was about 49.3 %.CONCLUSION: C2-ceramide can exert obvious cytotoxic effect in C6 cell through inducing typical early apoptosis of C6 cells, and it suggest that the enhancement of the level of ceramide might offer a novel approach to cancer chemotherapy.

Key words: C2-ceramide, C6, Annexin V/PI, early apoptosis

中图分类号:

R966

祁红, 刘艳梅, 吴兴军, 史桂英, 陈红专. C2-神经酰胺诱导大鼠脑胶质瘤细胞C6 的早期凋亡作用[J]. 中国临床药理学与治疗学, 2004, 9(12): 1349-1352.

QI Hong, LIU Yang-Mei, WU Xing-Jun, SHI Gui-Ying, CHEN Hong-Zhuan. Induction effects of C2-ceramide on early apoptosis in rat glioma cell C6[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(12): 1349-1352.

参考文献

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