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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (12): 1377-1380.

• 研究原著 • 上一篇    下一篇

重组人白细胞介素10 对角朊细胞增殖及产生细胞因子的影响

陈飞虎, 陈敏珠1, 李俊, 汪光宝, 智强2   

  1. 安徽医科大学药学院, 1临床药理研究所, 合肥 230032, 安徽;
    2安徽桑尼生物技术研究所, 合肥 230032, 安徽
  • 收稿日期:2004-07-08 修回日期:2004-12-06 出版日期:2004-12-26 发布日期:2020-11-19
  • 通讯作者: 陈飞虎, 男, 博士, 教授, 博士研究生导师, 研究方向:抗炎免疫药理与肿瘤药理。Tel:0551-5161116 E-mail:cfhchina@sohu.com

Effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cytokines production of normal human keratinocytes

CHEN Fei-Hu, CHEN Ming-Zhu1, LI Jun, ZHI Qiang2   

  1. Department of Pharmacy, 1Department of Clinical Pharmacology, AnhuiMedical University, Hefei 230032, Anhui, China;
    2Anhui Nisang Institute of Biology Technology, Hefei 230032, Anhui, China
  • Received:2004-07-08 Revised:2004-12-06 Online:2004-12-26 Published:2020-11-19

摘要: 目的: 研究重组人白细胞介素10(rhIL-10) 对无血清培养的角朊细胞增殖和产生细胞因子的影响, 探讨其治疗银屑病的作用机制。方法: 用四甲基偶氮唑蓝比色法(MTT) 测定其对细胞增殖的影响;小鼠胸腺细胞增殖法检测白细胞介素1(IL-1);ELISA 法检测白细胞介素6(IL-6)、白细胞介素8(IL-8) 。结果: 重组人白细胞介素10 抑制角朊细胞增殖与IL-1、IL-6 及IL-8 的分泌, 并呈剂量依赖关系。结论: 重组人白细胞介素10 抑制角朊细胞与细胞因子分泌, 可能是治疗银屑病的作用机理之一。

关键词: 重组人白细胞介素10, 角朊细胞, 细胞因子, 银屑病

Abstract: AIM: To investigate the effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cytokines production of serum-free cultured normal human keratinocytes stimulated by PMA and lipopolysaccharides(LPS).METHODS: The influences of rhIL-10 on the proliferation of serum-free cultured normal human keratinocytes were assayed by MTT.Interleukin-1, interleukin-6, and interleukin-8 was detected by Enzymelinked immunosorbent assay (ELISA).RESULTS: The rhIL-10 significantly inhibited the proliferation and the production of IL-1, IL-6 and IL-8 by KC which was activation by PMA LPS with dose-dependent manner.CONCLUSION: The rhIL-10 inhibits the proliferation and cytokines production of serum-free cultured normal human keratinocytes.The anti-psoriatic effects of rhIL-10 may be related to inhibiting hyperplasia of epidermal cells, promoting the formation of granular cells and possessing combined anti-inflammatory and immunomodulatory activity.

Key words: recombinant human interleukin-10, keratinocytes, cytokines, psoriatic

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