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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (12): 1381-1387.

• 研究原著 • 上一篇    下一篇

硝普钠抑制K562 细胞增殖并诱导细胞凋亡的实验研究

周永列, 吕亚萍1, 邱莲女, 何国浓2, 林惠君, 王文松   

  1. 浙江省人民医院中心实验室, 杭州 310014, 浙江;
    1浙江工业大学药学院, 杭州 310014, 浙江;
    2浙江中医学院生命科学系, 杭州 310053, 浙江
  • 收稿日期:2004-09-29 修回日期:2004-11-14 出版日期:2004-12-26 发布日期:2020-11-19
  • 通讯作者: 周永列, 男, 大学本科, 副主任医师, 副教授, 研究方向:实验血液学。Tel:0571-239988-3825 E-mail:zyl@zjxyjy.com
  • 基金资助:
    浙江省医学科学研究基金资助项目(No2002A0010)

Effects of sodium nitroprusside on proliferation inhibition and apoptosis in K562 cell line

ZHOU Yong-Lie, LV Ya-Ping1, QIU Lian-Nu, HE Guo-Nong2, LIN Hui-Jun, WANG Wen-Song   

  1. Central Laboratory, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China;
    1Colleage of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China;
    2Life Science Department of Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang, China
  • Received:2004-09-29 Revised:2004-11-14 Online:2004-12-26 Published:2020-11-19

摘要: 目的: 观察外源性一氧化氮(NO) 供体硝普钠对K562 细胞增殖抑制及诱导细胞凋亡作用。方法: 将不同浓度的硝普钠与K562 细胞在体外培养, 观察其作用时间效应和剂量效应;用活细胞计数、MTT 法观察硝普钠对K562 细胞增殖的抑制作用;用DNA凝胶电泳、DNA 含量及细胞周期分析、Annexin-V/PI双标记和DNA 片段原位末端标记法等分析细胞凋亡。同时设高铁氰化钾(PFC) 对照组和空白对照组。结果: NO 能抑制K562 细胞生长, 并在一定的剂量范围内呈现作用时间和剂量的量效关系, 大部分细胞阻滞于G0/G1 期;K562 细胞与硝普钠作用后出现典型的细胞形态改变, DNA 片断化, 亚G1 峰检出并显著增加, Annexin V/PI 和DNA 片段原位末端标记表达增加等均证实NO 能诱导K562 细胞凋亡。而对照组并无此类变化。结论: NO 通过阻滞G0/G1期细胞显著抑制K562 细胞的增殖, 并有很强的致凋亡作用。

关键词: 硝普钠/一氧化氮, 细胞株, K562, 增殖, 细胞凋亡

Abstract: AIM: To study the effects of nitric oxide donor sodium nitroprusside on proliferation inhibition and apoptosis in K562 human leukemia cell line.METHODS: The different concentration of sodium nitroprusside and K562 cell were cultivated at the different time in vitro.The proliferation inhibition was analyzed by MTT assay and alive cell count.Cell apoptosis was analyzed by cell morphology, DNA agarose gel electrophoresis, DNA content, and Annexin V/PI labeling method.The TDTmediated dUTP nick end labeling (TUNEL) assay was used to quantitate the cell apoptosis in situ.The PFC and the blank were used as controls.RESULTS: NO inhibited K562 cell proliferation within a certain range of treating time and dosage, and a majority of K562 cells were arrested in G0/G1 phase.The K562 cells apoptosis was confirmed by type cell morphology, DNA fragment, sub-G1 phase, TUNEL and Annexin V/PI labeling method with a time and dosage relationship, and the control group had no change like this.CONCLUTION: NO can suppress proliferation of K562 cell line by arresting G0/G1 phase and trigger apoptosis of the line.

Key words: sodium nitroprusside, cell line, K562, proliferation, apoptosis

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