转染外源性白细胞介素6 基因增强乳腺癌细胞株MCF-7 表达肿瘤相关抗原

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (12): 1408-1410.

转染外源性白细胞介素6 基因增强乳腺癌细胞株MCF-7 表达肿瘤相关抗原

陈方正, 江贤鹏¹, 陈淼², Jonathan FH¹, Robert LE¹, 姜希凌², 李欣燕²

江苏省中西医结合医院急诊外科, 南京 210000, 江苏;
¹The Elliott Breast Research and Treatment Center, Baton Rouge, LA 70816, USA;
²中国药科大学江苏省药代动力学重点实验室, 南京 210000, 江苏

收稿日期:2004-01-06 修回日期:2004-11-05 出版日期:2004-12-26 发布日期:2020-11-19
通讯作者: 李欣燕, 女, 博士, 研究方向:基因治疗。
作者简介:陈方正, 男, 医学学士, 主治医师。

Increased expression of tumor-associated antigen after interleukin 6(IL-6) mediated transfection in breast cancer cells

Chen Fang-Zheng, Jiang Xian-Peng¹, Duan Jin-Ao, Chen Miao², Jonathan FH¹, Robert LE¹, Jiang Xi-Ling², Li Xin-Yan²

Jiangsu Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing 210000, J iangsu, China;
¹the Elliott Breast Research and Treatment Center, Baton Rouge, LA 70816, USA;
²Key Laboratory of Pharmacokinetics, China Pharmaceutical University, Nanjing 210000, Jiangsu, China

Received:2004-01-06 Revised:2004-11-05 Online:2004-12-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 探索白细胞介素6(interleukin 6, IL-6)增强乳腺癌细胞表达乳腺癌抗原(CA15-3) 和癌胚抗原(CEA) 的作用。方法: 将含IL-6 蛋白编码顺序1176 bp cDNA 插入Pci-neo 哺乳动物表达载体。将重组载体转染MCF-7 乳腺癌细胞, 采用ELASA 方法测定细胞培养上清液内IL-6 浓度, 用MEIA(micropartical enzyme immunoassay) 方法测定上清液中肿瘤相关抗原CA15-3、CEA 和CA125 。结果: 含有外源IL-6基因的MCF-7 细胞分泌IL-6 浓度(338.5±22.6 pg·10^-6细胞) 明显高于父本没有含外源基因的MCF-7 细胞(25.4±4.6 pg·10^-6 细胞) 和仅含空载体Pci-neo 的MCF-7 细胞(19.6±3.0 pg·10^-6 细胞) (P<0.01) 。细胞培养d 3 后, 带外源IL-6 基因的MCF-7 细胞培养上清液中CA15-3 和CA125 水平(分别为14.9±2.3 和38.8±5.1 μg·10^-6细胞) 明显高于父本(分别为6.6±1.5 和10.0±1.6 μg·10^-6 细胞) 和空载体Pci-neo 的MCF-7 细胞(分别为3.4±0.7 和14.6±2.2 μg·10^-6 细胞, P <0.05) 。而转染IL-6 基因没有明显改变CEA 表达(P>0.05) 。结论: IL-6 具有诱导肿瘤相关抗原的表达和增强肿瘤细胞的免疫原性的作用, 提示IL-6 可能增强机体对肿瘤的免疫反应性。

关键词: 白细胞介素6, 乳腺癌细胞, 乳腺癌抗原, 癌胚抗原

Abstract: AIM: To investigate the effects of interleukin 6(IL-6) on the expression of breast cancer antigen (CA15-3) and carcinoembryonic antigen (CEA) in the breast cancer cells.METHODS: 1176 bp cDNA that contained the IL-6 protein encoding sequence was inserted into the Pci-neo mammalian expression vector, which contains a neomycin resistance selective marker.The vector was introduced into MCF-7 breast cancer cells by lipofection.Secretion of IL-6 in the culture media was measured by ELISA and the concentrations of CA15-3, CEA and CA125 in the culture media were quantitated by micropartical enzyme immunoassay (MEIA).RESULTS: The secretion of IL-6 (338.5±22.6 pg·10^-6 cells) in MCF-7 cells that contained the exogenous IL-6 gene significantly higher than that in the control parental MCF-7cells (25.4±4.6 pg·10^-6 cells) or the MCF-7 cells which only contained the Pci-neo mammalian expression vector (19.6±3.0 pg·10^-6 cells, P <0.01).After three days of incubation, the level of CA15-3 in the MCF-7 cells which contained the exogenous IL-6 gene (14.9±2.3 pg·10^-6 cells) was higher than that in the parental MCF-7 cells (6.6±1.5 pg·10^-6 cells) or the MCF-7 cells which only contained the Pci-neo vector (3.4±0.07 pg·10^-6 cells, P <0.05).The CA125 in the MCF-7 cells (38.8±5.1 pg·10^-6 cells) which contained the exogenous IL-6 gene significantly higher than that in the parental MCF-7 cells (10.0±1.6 pg·10^-6 cells) or the MCF-7 cells which only contained the Pcineo vector (14.6±2.2 pg·10^-6 cells, P <0.01 or P < 0.05).But the transfer of the exogenous IL-6 gene had not significantly increased the concentration of CEA in the culture medium compared to that in the control MCF-7 cells (P>0.05).CONCLUSION: IL-6 can induce the expression of tumor associated antigen and improve the antigenicity of tumor cells.And it suggest that IL-6maybe result in an increased immunological response.

Key words: interleukin 6, breast cancer cells, breast cancer antigen, carcinoembryonic antigen

中图分类号:

R966

陈方正, 江贤鹏, 陈淼, Jonathan FH, Robert LE, 姜希凌, 李欣燕. 转染外源性白细胞介素6 基因增强乳腺癌细胞株MCF-7 表达肿瘤相关抗原[J]. 中国临床药理学与治疗学, 2004, 9(12): 1408-1410.

Chen Fang-Zheng, Jiang Xian-Peng, Duan Jin-Ao, Chen Miao, Jonathan FH, Robert LE, Jiang Xi-Ling, Li Xin-Yan. Increased expression of tumor-associated antigen after interleukin 6(IL-6) mediated transfection in breast cancer cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(12): 1408-1410.

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