盐酸埃他卡林对大鼠海马神经元谷氨酸受体功能及突触活动的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 130-135.

盐酸埃他卡林对大鼠海马神经元谷氨酸受体功能及突触活动的影响

张颖丽, 汪海

军事医学科学院毒物药物研究所, 北京 100850

收稿日期:2003-07-11 修回日期:2003-08-22 出版日期:2004-02-26 发布日期:2020-11-16
通讯作者: 汪海,男, 医学博士, 研究员, 博士生导师, 主要从事心血管药理和新药研究工作。Tel:010-66932651 Fax:010-68211656 E-mail: wh@nic.bmi.ac.cn
作者简介:张颖丽, 女, 博士研究生。Tel:010-66874608 　E-mail: yingliz@yahoo.com
基金资助:
全军医药卫生科技基金重点项目(№01Z025)

Effects of iptakalim hydrochloride on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons

ZHANG Ying-Li, WANG Hai

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China

Received:2003-07-11 Revised:2003-08-22 Online:2004-02-26 Published:2020-11-16

摘要/Abstract

摘要： 目的: 研究盐酸埃他卡林(Ipt) 对脑神经元谷氨酸受体功能及突触活动的影响。方法: 采用原代培养的大鼠海马神经元, 应用膜片钳全细胞记录技术, 记录Ipt 对培养的海马神经元谷氨酸或天冬氨酸(NMDA) 诱发电流及神经元突触后电流的影响。结果: Ipt (1 ~ 100 μmol·L^-1) 可浓度依赖性地对抗培养的海马神经元谷氨酸或NMDA 诱发电流, 并为ATP敏感性钾通道拮抗剂格列本脲30 μmol·L^-1所对抗。Ipt 抑制培养的海马神经元之间突触联系形成的自发兴奋性突触后电流, 降低其发放频率, 抑制其电流幅度;但对微小兴奋性突触后电流无显著性影响。结论: Ipt 可阻断脑神经元谷氨酸受体功能, 抑制脑神经元谷氨酸的兴奋性突触传递, 其作用与ATP 敏感性钾通道相关。

关键词: 盐酸埃他卡林, 海马, 神经元, 谷氨酸, NMDA, 膜片钳

Abstract: AIM: To investigate the effects of iptakalim hydrochloride (Ipt) on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons.METHODS: The cultured hippocampal neurons from neonatal rats were served as preparation.The effects of Ipt on glutamate receptors and spontaneous synaptic activities were observed by using whole-cell recording technique .RESULTS: Different concentrations of Ipt (1-100 μmol·L^-1) suppressed the glutamate and NMDA induced currents of rat hippocampal neurons in a dose-dependent manner, and these inhibitory effects were antagonized by K_ATP blocker glibenclamide (30 μmol·L^-1).Ipt inhibited the sEPSCs in cultured rat hippocampus neurons, and decreased both the frequency and amplitude, with no effect on mEPSCs.CONCLUSION: Ipt can block the function of glutamate receptor and attenuate the glutamatergic transmission in cerebral neurons, and these effects may be related with K_ATP.

Key words: iptakalim, hippocampus, neuron, glutamate, NMDA, patch clamp

中图分类号:

R965
R971

张颖丽, 汪海. 盐酸埃他卡林对大鼠海马神经元谷氨酸受体功能及突触活动的影响[J]. 中国临床药理学与治疗学, 2004, 9(2): 130-135.

ZHANG Ying-Li, WANG Hai. Effects of iptakalim hydrochloride on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(2): 130-135.

参考文献

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