氧化型低密度脂蛋白对PC12 细胞的毒性作用

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 166-170.

氧化型低密度脂蛋白对PC12 细胞的毒性作用

曹莉, 尹鸿操¹, 周礼¹, 王拥军

首都医科大学附属天坛医院神经内科, 北京 100050;
¹中国医学科学院基础医学研究所病理室, 北京 100005

收稿日期:2003-06-07 修回日期:2003-12-02 出版日期:2004-02-26 发布日期:2020-11-16
通讯作者: 曹莉,女, 副主任医师, 医学博士, 现在北京安贞医院神经内科。Tel:010-64412431-2659 E-mail: Li_cao@hotmail.com

Cytotoxicity of oxidized low-density lipoprotein on PC12 cells

CAO Li, YIN Hong-Cao¹, ZHOU Li¹, WANG Yong-Jun

Department of Neurology, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050;
¹Department of Pathology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China

Received:2003-06-07 Revised:2003-12-02 Online:2004-02-26 Published:2020-11-16

摘要/Abstract

摘要： 目的: 探讨氧化型低密度脂蛋白(oxLDL) 对 PC12 细胞的毒性作用。方法: 通过HE 染色、透射电镜、MTT 实验、LDH 释放实验、TUNEL 实验及 Caspase-3 测定来观察oxLDL 对PC12 细胞形态、存活率、细胞膜通透性、细胞核及Caspase-3 活性的影响。结果: 透射电镜及光镜下oxLDL 作用的PC12 细胞以坏死为主并伴少量凋亡细胞。细胞存活率随着ox-LDL浓度及作用时间增加而下降;LDH 释放率、TUNEL 阳性细胞数及Caspase-3 活性随着浓度增高而增高;LDL 对上述各项指标无影响。结论: oxLDL通过Caspase-3 途径致PC12 细胞的毒性作用主要以坏死细胞为主并伴少量凋亡细胞, 并呈量效与时效关系;而LDL 无细胞毒性作用。

关键词: 氧化型低密度脂蛋白, PC12 细胞, 细胞毒性, Caspase-3

Abstract: AIM: To investigate the cytotoxicity of oxidized low-density lipoprotein (oxLDL) on PC12 cells.METHODS: PC12 cells were applied to be a neuron model and exposed to oxLDL.Cell injury was evaluated by cell viability (MTT assay), cell lysis (LDH assay), DNA fragmentation (TUNEL assay) and Caspase-3 activity (Caspase assay).RESULTS: Most of PC12 cells treated with oxLDL showed cell lysis changes and a few cells showed apoptotic changes, observed with electromicroscopically .The cell viability decreased with the concentration of oxLDL increasing.As the concentration of oxLDL increased, LDH release, DNA fragmentation and Caspase-3 activity also increased.However, there was no significant alteration in untreated control cultures and LDL control.CONCLUSION: oxLDL, but not native LDL, resultes in a dose- and time-dependent neuron death including cell lysis and apoptosis in which Caspases-3 participates.

Key words: oxidized low-density lipoprotein, PC12 cells, cytotoxicity

中图分类号:

R963

曹莉, 尹鸿操, 周礼, 王拥军. 氧化型低密度脂蛋白对PC12 细胞的毒性作用[J]. 中国临床药理学与治疗学, 2004, 9(2): 166-170.

CAO Li, YIN Hong-Cao, ZHOU Li, WANG Yong-Jun. Cytotoxicity of oxidized low-density lipoprotein on PC12 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(2): 166-170.

参考文献

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