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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 177-179.

• 研究原著 • 上一篇    下一篇

3 种CAg 检测方法评价吡喹酮治疗日本血吸虫病疗效的比较

尹东, 陈红根, 许雪萍, 衣方誉, 姜唯声, 曾小军, 汪亚群, 高晓晖   

  1. 江西省寄生虫病防治研究所, 南昌330046, 江西
  • 收稿日期:2003-07-30 修回日期:2003-09-15 出版日期:2004-02-26 发布日期:2020-11-16
  • 通讯作者: 尹东,女, 副主任医师, 研究方向:寄生虫病免疫学诊断。Tel:0791-6204520

Comparison of three assays of CAg detection on evaluation of praziquantel efficacy in treatment with patients with schistosomiasis Japonica

YIN Dong, CHEN Hong-Gen, XU Xue-Ping, YI Fang-Yi, JIANG Wei-Sheng, ZHENG Xiao-Jun, WANG Ya-Qun, GAO Xiao-HeiJiangxi   

  1. Provincial Institute of Parasitic Diseases, Nanchang 330046, Jiangxi, China Jiangxi
  • Received:2003-07-30 Revised:2003-09-15 Online:2004-02-26 Published:2020-11-16

摘要: 目的: 探讨3 种不同的循环抗原(CAg) 检测方法在日本血吸虫病人经吡喹酮治疗后疗效考核的应用价值。方法: 采用单克隆抗体(MAb) JPG3, 分别建立了Dot-ELISA 、Sandwich-ELISA 和R-IHA 3 种CAg检测方法, 用吡喹酮(50 mg·kg-1, 顿服) 治疗日本血吸虫病人, 进行治疗后不同时期的同步血清学检测,并评估检测结果。结果: 吡喹酮治疗后半年, 上述3种检测方法的CAg 阴转率分别为75.8 %、72.6 %和43.5 %(P<0.01), 治疗后1 年分别为83.9 %、88.7 %和71.0 %(P<0.05) ;治疗后2 年分别为87.1 %、95.2 %和93.5 %(P >0.05) ;CAg 阳性病例的平均抗原滴度倒数(GMRT) 随治疗后时间的延长逐步下降, 治疗后2 年三种方法均达到各自的最低阳性稀释度。结论: 考核吡喹酮治疗日本血吸虫病人近期(半年或1 年) 疗效, Dot-ELISA 和Sandwich-ELISA 均较好, 但后者在结果判读上较前者更为客观;考核远期(2 年) 疗效, 3 种方法的检测结果无显著性差别;反映CAg 水平的GMRT 在一定程度上亦能评估吡喹酮之疗效。

关键词: 吡喹酮, 血吸虫病, 循环抗原, 疗效考核, 单克隆抗体

Abstract: AIM: To compare 3 different methods of detecting the circulating antigen (CAg) to evaluate the chemotherapeutic effect in treatment with patients with schistosomiasis Japonica by praziquantel.METHODS: Serum CAg was detected in patients by Dot-ELISA, Sandwich-ELISA and R-IHA, based on monoclonal antibody (MAb) JPG3.The reverse negative rates were analyzed at six months, one year and two years after treatment with praziquantel 50 mg·kg-1 (one dosage).RESULTS: The reverse negative rates of CAg in serum treated with praziquantel were 75.8 %, 72.6 %and 43.5 %at six months (P<0.01), 83.9 %, 88.7 % and 71.0 % at one year (P<0.05), and 87.1 %, 95.2 % and 93.5 % at two years (P >0.05) by Dot-ELISA, Sandwich-ELISA and R-IHA, respectively.The geometric mean reciprocal titer (GMRT) in positive individuals declined constantly at six months, one year and two years.CONCLUSION: Dot-ELISA and Sandwich-ELISA are better than R-IHA in evaluation of chemotherapy effect of praziquantel at six months and one year, whereas Sandwich-ELISA is more objective than Dot-ELISA for reading results.All of three assays have no significant difference after two years of treatment.TheGMRT that reflects circulating antigen level can also assess the cure after chemotherapy with praziquantel to a certain extent.

Key words: praziquantel, schistosomiasis, circulating antigen, evaluation of chemotherapy effect, monoclonal antibody

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