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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 197-200.

• 研究原著 • 上一篇    下一篇

川芎嗪对儿茶酚胺诱导大鼠心肌损伤时Bcl-2和Fas 蛋白表达的影响

陆一敏, 万福生1, 唐三保   

  1. 江西省人民医院药剂科, 南昌 330006, 江西;
    1江西医学院生化与分子生物学教研室, 南昌 330006, 江西
  • 收稿日期:2003-08-11 修回日期:2003-10-22 出版日期:2004-02-26 发布日期:2020-11-16
  • 通讯作者: 万福生,男, 硕士, 教授, 硕士生导师, 研究方向:心血管分子生物学。E-mail: wanfusheng2003 @yahoo.com.cn
  • 作者简介:陆一敏, 女, 大学本科, 主管药师, 研究方向:心血管药理。
  • 基金资助:
    江西省自然科学基金资助题(NO.0040054)

Effects of tetramethylpyrazine on expression of Bcl-2 and Fas proteins in myocardial injury induced by catecholamine in rats

LU Yi-Min, WAN Fu-Sheng1, TANG San-Bao   

  1. Department of Pharmacy, Jiangxi Provincial Hospital, Nanchang 330006, Jiangxi, China;
    1Department of Biochemistry and Molecular Biology, Jiangxi Medical College, Nanchang 330006, Jiangxi, China
  • Received:2003-08-11 Revised:2003-10-22 Online:2004-02-26 Published:2020-11-16

摘要: 目的: 观察川芎嗪对儿茶酚胺诱导大鼠心肌损伤时心肌细胞Bcl-2 和Fas 蛋白表达的影响。方法: 采用皮下多点注射异丙肾上腺素(ISO,5 mg·kg-1) 每天1 次, 连续3 d, 诱导大鼠心肌损伤,采用免疫组化方法检测Bcl-2 和Fas 蛋白的水平, 并利用图像分析系统分析蛋白阳性表达区域平均光密度值。结果: 损伤组和川芎嗪保护组心肌细胞Bcl-2蛋白表达较对照组均显著升高(P<0.01), 损伤组Fas 蛋白水平较对照组也显著升高(P<0.01), 川芎嗪保护组的Fas 蛋白水平较损伤组显著下降(P<0.01), 且Bcl-2 Fas 比值也较损伤组和对照组明显增加。川芎嗪保护组的病理损伤程度明显低于损伤组。结论: 川芎嗪可抑制儿茶酚胺诱导的心肌损伤时心肌细胞中促凋亡基因Fas 的表达, 提高心肌细胞中Bcl-2 Fas 比值。

关键词: 川芎嗪, 心肌损伤, Bcl-2, Fas, 凋亡, 儿茶酚胺

Abstract: AIM: To observe the influence of tetramethylpyrazine on the expression of Bcl-2 and Fas proteins in myocardial injury induced by catecholamine in rats.METHODS: 36 Wistar rats were divided into 3 groups: cardiac injury group (n =12), tetramethylpyrazine treated group (n =12) and control group (n =12).The rat cardiac injury was induced by the subcutaneous injection of isoproterenol (ISO, 5 mg·kg-1·d-1).Bcl-2 and Fas protein was identified by immunohistochemical technique and the mean optical density (OD) values of the positive fields of protein expression were quantitatively examined by image analysis system.RESULTS: The expression of Bcl-2 protein was increased significantly in the cardiac injury group and tetramethylpyrazine treated group as compared with that of the control group (P<0.01).There were no significant difference in the Bcl-2 protein expression between the tetramethylpyrazine treated group and cardiac injury group (P >0.05), but the expression of Fas protein was decreased significantly in the tetramthylpyrazine treated group as compared with that of the cardiac injury group (P<0.05).The protein expression ratio of Bcl-2 Fas was increased significantly in the tetramethylpyrazine treated group as compared with those of the cardiac injury group and the control group.The myocardium pathological damage was decreased significantly in the tetramethylpyrazine treated group.CONCLUSION: Tetramethylpyrazine can significantly inhibit the expression of pro-apoptotic Fas protein and raise the ratio of Bcl-2 Fas protein cardiomyocytes.

Key words: tetramethylpyrazine, myocardium injury, Bcl-2, Fas, apoptosis, catecholamine

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