欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 226-229.

• 研究原著 • 上一篇    下一篇

泛影葡胺对糖尿病肾病大鼠心肌细胞内皮素受体A 表达的影响

李景荣, 熊术道1, 汪延辉2, 吴建波1, 温博贵3   

  1. 温州医学院附属第一医院急诊科, 1医科所, 2肾内科, 温州325000, 浙江;
    3汕头大学医学院分子生物学研究室, 汕头 510031, 广东
  • 收稿日期:2003-07-22 修回日期:2003-10-14 出版日期:2004-02-26 发布日期:2020-11-16
  • 通讯作者: 李景荣,女, 医学硕士, 主治医师, 研究方向:冠心病的基础及临床。Tel:0577-88863266  E-mail: lijr1@yahoo.com

Effects of diatrizoate on endothelin receptor A in cardiac myocytes of diabetic nephropathy SD rats

LI Jing-Rong, XIONG Shu-Dao1, WANG Yuan-Hui2, WU Jian-Bo1, WEN Bo-Gui3   

  1. Department of Emergency, 1Medical Science Institute, 2Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China;
    3Department of Molecular Biology, Medical College of Shantou University, Shantou 515031, Guangdong, China
  • Received:2003-07-22 Revised:2003-10-14 Online:2004-02-26 Published:2020-11-16

摘要: 目的: 观察造影剂泛影葡胺对糖尿病肾病大鼠心肌细胞内皮素受体A (endothelin receptor A,ETAR) 表达的影响。方法: 应用链脲佐菌素(STZ) 腹腔注射建立SD 大鼠糖尿病肾病模型。设对照组、模型组、泛影葡胺组, BQ123 +泛影葡胺组, 利用RT-PCR 和western blot 方法检测心肌组织ETAR 表达的变化。结果: 与对照组比较, 各组糖尿病大鼠心肌ETAR 表达上调, CM 组上调尤为明显(P<0.05),注射BQ123 后, 可见其表达下调。结论: 有糖尿病肾病基础病史时, 泛影葡胺可引起心肌组织ETAR表达变化, 从而影响心脏功能;预防性使用选择性ETAR 拮抗剂BQ123, 可下调ETAR 蛋白表达, 改善心脏功能。

关键词: 造影剂, 泛影葡胺, 糖尿病肾病, 内皮素受体A, Western blot, RT-PCR

Abstract: AIM: To investigate the effects of diatrizoate on the expression of endothelin receptor A (ETAR) in cardiac myocyte of diabetic nephropathy SD rats.METHEDS: 32 male SD rats (220-280 g) were randomly divided into four groups:8 normal control rats (Con group) with sham surgeon operation ;the other 24 were diabetic rats which were induced by intraperitoneal streptozotocin (35 mg·kg-1 body weight) at one dose after they were uninephrectomized for 2 weeks.At the end of the eighth week, the diabetic rats were divided into 3 groups at random:(DM group) ;diabetic rats plus diatrizoate injection (10 ml·kg-1) through tail vein of rats (CM group) ;BQ123 treated group (BQ group) which were injected the BQ123 (1 mg·kg-1) before being injected the diatrizoate, while the Con group were only given the sterile water.ETARwas examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot.RESULTS: Both RT-PCR and Western blot analysis indicated that ETAR had been upregulated in CM, DM and BQ groups compared with that in Con group (P<0.05) ;especially in CM group.BQ123 could downregulate the expression of ETAR gene induced by diatrizoate .CONCLUSION: Increasing ETAR may affect cardiac function induced by radiocontrast media diatrizoate under diabetic nephropathy conditions.However, BQ123 can downregulate the expression of ETAR gene induced by radiocontrast media diatrizoate and improve cardiac function.

Key words: radiocontrast media, diatrizoate, diabetes mellitus nephropathy, ETAR, Western blot, RT-PCR

中图分类号: