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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (5): 504-509.

• 研究原著 • 上一篇    下一篇

灵菌红素对人胰腺癌细胞增殖抑制的实验研究

沈亚领, 刘建文, 魏东芝, 陶金莉, 李柯, 张靖   

  1. 华东理工大学生物反应器工程国家重点实验室, 上海200237
  • 收稿日期:2003-09-08 修回日期:2003-11-04 发布日期:2020-11-22
  • 通讯作者: 刘建文, 男, 博士, 教授, 硕士生导师, 主要从事抗肿瘤药物及生物活性物质的功效研究。Tel:021-64253715 E-mail:liujian@ecust.edu.cn
  • 作者简介:沈亚领, 男, 副教授, 硕士生导师, 主要从事基因工程药物及生物活性物质的研发。Tel:021-64253156 E-mail:yalings @online.sh.cn

Inhibition of prodigiosin on multiplication of human malignant pancreas cancer cells

SHEN Ya-Lin, LIU Jian-Wen, WEI Dong-zhi, TAO Jin-Li, LI Ke, ZHANG Jing   

  1. State Key Laboratory of Bioreactor Engineer, Institute of New World Biotechnology, East China University of Science and Technology, Shanghai 200237, China
  • Received:2003-09-08 Revised:2003-11-04 Published:2020-11-22

摘要: 目的 观察从生物工程技术得到的灵菌红素对人胰腺癌8898 细胞增殖抑制的药效学作用, 并探讨其作用的部分机理。 方法 用MTT 法测定8898细胞的存活率和抑制率, 用流式细胞仪分析细胞的周期变化和凋亡细胞的百分比, 用HPLC 检测人胰腺癌8898 细胞内灵菌红素的浓度, 用琼脂糖凝胶电泳对DNA 段片化进行分析。 结果 灵菌红素5 mg·L-1的培养液中8898 细胞出现凋亡早期的形态学特征, 半数抑制浓度(IC50) 为30 mg·L-1。而3T3 细胞却未显示出该作用。研究表明灵菌红素抑制细胞的增殖, 与抑制S 期细胞的DNA 复制及调控细胞的增殖周期相关。同时, 细胞凋亡的产生与实验药物呈正相关。HPLC 结果显示, 灵菌红素的药理学作用与细胞内药物浓度相关。 结论 灵菌红素能有效的进入细胞内, 抑制细胞的增殖, 其机理与诱导细胞凋亡和调控细胞的增殖周期相关。

关键词: 灵菌红素, 人胰腺癌, 细胞凋亡, 增殖周期, 流式细胞分析术

Abstract: AIM: To investigate the inhibitive effects of prodigiosin on the multiplication of human malignant pancreas cancer cell 8898, and its mechanism. METHODS: MTT assay was used to measure the rate of viability and inhibition of 8898 cells;the change of cell cycle and the percent of apoptosis were analyzed by FCM;the concentration of prodigiosin in 8898 cells was measured HPLC;and the fragmented DNA was analyzed by agarose gel electrophoresis. RESULTS: In the culture medium containing 5 mg·L-1 prodigiosin, 8898 cells appeared the morphological character of early stage of apoptosis.Half inhibition concentration (IC50) was 30 mg·L-1.But to 3T3 cells the drug had no obvious effect.The inhibitive effect of prodigiosin on multiplication of cancer cells was related to the inhibiting of DNA replication in S stage and regulating of cell cycle.The results of HPLC showed that pharmacological effect of prodigiosin dependent on drug concentration in cells. CONCLUSION: Prodigiosin can enter cells and inhibit the multiplication of cells.The mechanism may be related to inducing apoptosis and regulating cell cycle.

Key words: prodigiosin, human pancreas cancer, apoptosis, proliferation cycle, FCM analytical method

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