花椒毒酚对豚鼠离体心房肌生理特性的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (5): 588-591.

花椒毒酚对豚鼠离体心房肌生理特性的影响

刘建新, 周青, 周俐, 连其深

赣南医学院药理教研究室, 赣州341000, 江西

收稿日期:2003-11-11 修回日期:2004-03-11 发布日期:2020-11-22
通讯作者: 刘建新, 男, 硕士, 讲师, 研究方向:中草药Tel:0797-8209174 　E-mai l:jianxingliu @yahoo.com.cn
基金资助:
江西省科技社会发展处课题(No20021231015)

Effects of xanthotoxol on physiological characteristics of isolated guinea pig atrium

LIU Jian-Xing, ZHOU Qing, ZHOU Li, LIAN Qi-Shen

Department of Pharmacology, Gannan Medical College, Ganzhou 341000, Jiangxi, China

Received:2003-11-11 Revised:2004-03-11 Published:2020-11-22

摘要/Abstract

摘要： 目的研究花椒毒酚对豚鼠离体心肌生理特性的影响。方法采用豚鼠离体心房, 测定花椒毒酚对豚鼠心房率, 心肌收缩力, 左心房的兴奋性的影响。结果在心肌收缩力的实验中, 给花椒毒酚20,40, 80 μmol·L^-1 15 min后, 左心房的收缩力分别为0.85、0.68、0.48 g, 与对照组比较, 花椒毒酚明显抑制豚鼠左心房肌的收缩力(P <0.05), 并呈浓度依赖性;在对心房率的实验中, 给花椒毒酚20,40 μmol·L^-1, 30 min 后, 离体右心房的频率从90.0 次·min^-1分别下降至60.2、38.7 次·min^-1, 与对照组比较有显著的统计学意义(P <0.05);花椒毒酚对豚鼠左心房的功能性不应期和左心房兴奋性无明显影响。结论花椒毒酚可以降低心房自律性、抑制心房收缩力, 其负性变频和负性变力效应可能与其抑制心肌细胞外钙内流和内钙释放有关。

关键词: 花椒毒酚, 香豆素类, 豚鼠心房肌, 功能性不应期, 收缩性

Abstract: AIM: To study the effects of xanthotoxol (XT) on physiological characteristics and its mechanism in isolated guinea pig atria. METHODS: It was determined by the contractile amplitude, excitability and the spontaneous beats in the right atria, respectively. RESULTS: In the experiment of contractile amplitude, after 15 min of administration of XT 20, 40 and 80 μmol·L^-1, the contractile force of left atria was 0.85, 0.68, and 0.48 g, respectively (P <0.05, compared with control group).It showed that XT could decrease the contractile force in concentration-dependent manner significantly.In the experiment of the sinus rates, after 30 min of administration of XT 20 and 40 μmol·L^-1, the fact that the sinus rates decreased from 90.0 min-1 to 60.2 min-1 and 38.7 min-1, respectively (P <0.05 vs solvent group) indicated that XT significantly reduced the sinus rates.In the experiments of the functional refractory period and excitability, no effects of XT were demonstrated. CONCLUSION: XT can decrease the contractile force and the spontaneous beats.The inotropic and chronotropic action of XT may be related to inhibiting Ca²⁺ influx from outside the cells and Ca 2+ release from calcium reservoir inside the cells.

Key words: xanthotoxol, coumarin, guinea pig auricle, functional refractory period, contractility

中图分类号:

R285.5
R337

刘建新, 周青, 周俐, 连其深. 花椒毒酚对豚鼠离体心房肌生理特性的影响[J]. 中国临床药理学与治疗学, 2004, 9(5): 588-591.

LIU Jian-Xing, ZHOU Qing, ZHOU Li, LIAN Qi-Shen. Effects of xanthotoxol on physiological characteristics of isolated guinea pig atrium[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(5): 588-591.

参考文献

1 上官珠, 连其深, 曾靖, 周青, 皮荣标, 刘建新, 等.花椒毒酚镇痛实验研究[J].中药新药与临床药理, 1997;8(1):45-7
2 连其深, 张志祖, 胡晓, 周俐, 上官珠, 叶和杨, 等.花椒毒酚抗炎作用的实验研究[J].中草药, 1998;29(2):102-4
3 连其深, 张志祖, 上官珠, 周俐, 皮荣标, 向仁德, 等.花椒毒酚抗实验性心律失常作用的研究[J].中草药, 1996;27(6):347-9
4 胡晓, 周俐, 连其深, 曾靖, 杨敬格, 赖飞, 等.花椒毒酚对家兔离体回肠的作用[J].中国中药杂志, 2001;26(1):56-8
5 胡晓, 连其深, 周俐, 赖飞, 周青, 叶和杨, 等.花椒毒酚对大鼠离体子宫平滑肌的作用[J].赣南医学院学报, 2000;20(2):98-101
6 向仁德, 傅晓红.蛇床子化学成份的研究(1) [J].中草药, 1984;15(9):14-6
7 Govier WC.The mechanism of the atrial refractory period change produced by ouabain[J].J Pharmacol Exp Ther, 1965; 148:100-11
8 袁昌齐.中国伞形科药用植物概要[J].中药通报, 1986;11(1):5-8
9 Sharova GP.Effect of pteryxin and dihydrosamidine on the electrical activity of rabbit hearts during a pituitrin coronary spasm [J].Chem Abs, 1973;7a:27316a
10 Sethi OP, Naik K.Xanthotoxol (XT)-a potent 5-HT antagonist [J].Indian J Physiol Pharmacol, 1979;23(2):142-3
11 徐叔云, 卞如濂, 陈修.药理实验方法学[M], 第3 版, 北京:人民卫生出版社, 2002:1182-3
12 丁吉新, 张世玲, 王如斌.关附乙酯对大鼠离体心房肌的影响[J].中国药理学报, 1997;13(6):541-3
13 Reuter N, Heeg E, Haller U.Beeinflussunger funktionellen Refrakarzeit under Kkkontraktionskaft eledtrisch gereizter Meesch-weinchenvorhofe durch Antiarrhythmica und β-Receptorenblocker[J].Naunyn Schmiedebergs Arch Pharmakol, 1971;268(3):323-32
14 Dalla NS, Pierce GN.Panagia V, Singal PK, Beamish RE. Calcium movements in relation to heart function[J].Basic Res Cardiol, 1982;77(2):117-23

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