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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (6): 662-665.

• 研究原著 • 上一篇    下一篇

阿霉素热化疗诱导A549 细胞凋亡及线粒体跨膜电位的变化

张继贤, 张启昆, 万毅新   

  1. 兰州医学院第二附属医院呼吸内科, 兰州730030, 甘肃
  • 收稿日期:2004-04-08 修回日期:2004-05-10 发布日期:2020-11-22
  • 作者简介:张继贤, 男, 硕士研究生, 医师, 研究方向:呼吸肿瘤。Tel:0931-3624402  E-mai l:jixianzhang2003 @yahoo.com.cn;张启昆, 男, 副教授, 主任医师, 硕士生导师, 研究方向:肺癌的中西医治疗。Tel:0931-8942408

Apoptosis induced by thermochemotherapy of adriamycin (ADM) and the change of mitochondial transmembrance potential in A549 cells

ZHANG Ji-Xian, ZHANG Qi-Kun, WAN Yi-Xin   

  1. Department of Respiratory Medicine, The Second Affiliated Hospital of Lanzhou Medical College, Lanzhou 730030, Gansu,China
  • Received:2004-04-08 Revised:2004-05-10 Published:2020-11-22

摘要: 目的 探讨阿霉素(adrimycin, ADM) 热化疗诱导人肺腺癌A549 细胞凋亡及对线粒体跨膜电位(Δψm) 的影响。 方法 不同浓度的阿霉素作用于体外培养的A549 细胞, 42.5 ℃ 作用30 min 后, 37 ℃继续培养, 在不同时间内检测。用电镜、MTT 法、流式细胞仪检测。 结果 阿霉素热化疗明显增强对A549 细胞的抑制作用, 细胞内阿霉素的浓度显著高于化疗组(P < 0.01);阿霉素浓度为1.0,2.0 mg·L-1时, 热化组与化疗组比较, 凋亡率增高,线粒体膜电位下降(P <0.01)。 结论 线粒体跨膜电位下降可能是ADM 热化疗诱导A549 细胞凋亡的关键。

关键词: 阿霉素, 热化疗, A549 细胞, 凋亡, 线粒体跨膜电位

Abstract: AIM: To investigate the effect of apoptosis and the change of mitochondial tansmembrane potential in A549 cells induced by thermochemotherapy of ADM. METHODS: ADM of different concentrations was directly applied to A549 cells cultivated in vitro.Cells maintained at 42.5 ℃for 30min. RESULTS: The inhibition of A549 cells was increased by thermochemotherapy of ADM significantly, and the cytoplasmic concentration of thermochemotherapy of ADM was significantly higher than that of chemotherapy (P <0.01).When it was 1.0 and 2.0 mg·L-1, apoptosis rate was increased and mitochondrial transmembrane potential was decreased by thermochemotherapy of ADM (P <0.01). CONCLUSION: The change of motochondial transmembrane potential was the key of apoptosis induced by thermochemotherapy of ADM in A549 cells.

Key words: adriamycin, thermochemotherapy, A549 cells, motochondial transmembrane potential

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