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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (6): 669-672.

• 研究原著 • 上一篇    下一篇

反相高效液相色谱法测定犬血浆中左氧氟沙星浓度及其犬体内药代动力学

姜希凌, 孙建国, 王广基, 李昊, 李鹏1, 何卉1   

  1. 中国药科大学药代动力学重点实验室, 南京210009, 江苏
  • 收稿日期:2004-01-26 修回日期:2004-04-12 发布日期:2020-11-22
  • 通讯作者: 王广基, 男, 博士, 教授, 博士生导师, 主要研究方向:药物代谢动力学。Tel:025-83271544  E-mai l:gjwang @cpu.edu.cn
  • 作者简介:姜希凌, 李昊, 硕士研究生。
    1李鹏, 何卉, 中国药科大学2003 届本科毕业生。
  • 基金资助:
    江苏省药代动力学重点实验室资助项目(NoBM2001201)

Determination of levofloxacin in dog plasma using RP-HPLC and its pharmacokinetics

JIANG Xi-Ling, SUN Jian-Guo, WANG Guang-Ji, LI Hao, LI Peng, HE Hui   

  1. Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu, China
  • Received:2004-01-26 Revised:2004-04-12 Published:2020-11-22

摘要: 目的 建立测定犬体内左氧氟沙星血药浓度的反相高效液相色谱法, 并测定其犬体内药代动力学。 方法 以环丙沙星为内标, 采用10 %高氯酸为沉淀剂处理犬血浆样品, 色谱柱为DIKMA DiamonsilTM C18, 5 m, 150 mm ×4.6 mm, 流动相为甲醇-PBS-三乙胺-异丙醇(33 ∶67 ∶0.14 ∶0.4, 用磷酸调pH为3.0), 流速1.2 ml·min-1, 柱温20 ℃, 荧光检测(λex=295 nm, λem=440 nm)。 结果 血药浓度线性范围0.25 ~ 50 mg·L-1(r=0.9998), 最低检测限浓度为0.02 mg·L-1 (S N >3), 绝对回收率为83.0 % ~85.6 %(n=5), 方法回收率为92.10 % ~ 106.77 %(n=5), 日内和日间RSD 分别为1.3 %~ 4.0 %和1.8 %~ 8.2 %(n=5)。 结论 本方法简便、灵敏、精密度高, 重现性好, 适用于左氧氟沙星犬体内药代动力学研究及生物等效性研究。

关键词: 左氧氟沙星, 反相高效液相, 血药浓度, 药代动力学

Abstract: AIM: To establish a HPLC method for the determination of levofloxacin in dog plasma and its pharmacokinetics in dogs. METHODS: The plasma protein was precipitated by the addition of 10 % perchloric acid.The resulting supernatant liquid was directly injected after centrifuge and dilution.The chromatography conditions were:DIKMA column (DiamonsilTM C18 5 μm, 150 mm ×4.6 mm), methanol-PBS-triethylamine-isopropanol (33 ∶67 ∶0, 14 ∶0.4, adjusted pH to 3.0 with H3PO4) as mobile phase with a flow rate of 1.2 ml·min-1.It was detected by fluorescence detector, and the detection wavelength was λex=295 nm and λem=440 nm.Ciprofloxacin was the internal standard. RESULTS: The linear range of calibration curve was within drug plasma concentrations of 0.25-50 mg·L-1 (r=0.9998), the detection limit was 0.02 mg·L-1 (S N > 3), the extraction recovery was 83.0 %-85.6 % (n=5), the method recovery was 92.10 %-106.77 %(n=5), the within-day RSD and between-day RSD were 1.3 %-4.0 % and 1.8 %-8.2 % (n=5), respectively. CONCLUSION: The method possesses merits of simplicity, sensitivity, good accuracy, high precision, and fine reproducibility, which is suitable for pharmacokinetic and bioavailability studies of levofloxacin in dogs.

Key words: levofloxacin, HPLC, drug concentration, pharmacokinetics

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