阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD44V6表达的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (8): 906-910.

阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD₄₄V₆表达的影响

高雪芹^{1, 2}, 韩金祥^{1, 2}, 张维东³, 宋守芹³, 朱波¹

¹卫生部生物技术药物重点实验室, 山东省医药生物技术研究中心, 济南250062, 山东;
²山东大学医学院, 济南250014, 山东;
³山东省医学科学院基础医学研究所, 济南250062, 山东

收稿日期:2004-05-26 修回日期:2004-07-14 出版日期:2004-08-26 发布日期:2020-11-20
通讯作者: 韩金祥,男,博士生导师,研究员,研究方向为基因工程与基因分析。Tel:0531-2919888 E-mail:jxhan@sdu.edu.cn
作者简介:高雪芹,女,博士生,研究员,主要从事肿瘤分子生物学研究。Tel:0531-2919608 E-mail:xueqingao666@163.com
基金资助:
山东省卫生厅青年基金资助项目(№1999CA2CACA1)

Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD₄₄V₆ in 3AO cells

GAO Xue-Qin^{1, 2}, HAN Jin-Xiang^{1, 2}, ZHANG Wei-Dong³, SONG Shou-Qin³, ZHU Bo¹

¹Shandong Medicinal and Biotechnology Center, Shandong Academy of Medical Sciences, Key Laboratory for Biotechdrug,Ministry of Health, Jinan 250062, Shangdong, China;
²Medical College of Shandong University, Jinan 250014, Shangdong, China;
³Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062,Shangdong, China

Received:2004-05-26 Revised:2004-07-14 Online:2004-08-26 Published:2020-11-20

摘要/Abstract

摘要： 目的: 研究阿司匹林、舒林酸对3AO 细胞环氧合酶-2(COX-2)、血管生长因子(VEGF)、CD₄₄V₆ 及层粘蛋白受体(LN-R)表达的影响。方法: 采用MTT方法观察阿司匹林、舒林酸在贴壁前后加入培养液中对3AO 细胞生长的影响。采用免疫细胞化学的方法研究两种药物对3AO 细胞COX-2、VEGF、CD₄₄V₆、LN-R 的影响。结果: MTT 实验显示阿司匹林、舒林酸不论在贴壁前还是后加入均可抑制3AO 卵巢癌细胞的生长,加药后调节pH 可减弱生长抑制作用但仍然可明显抑制肿瘤细胞的生长。阿司匹林和舒林酸对COX-2、VEGF 的表达也有抑制作用,但对CD₄₄V₆ 及LN-R 的表达无明显影响。结论: 抑制COX-2 的表达可以下调VEGF 的表达,从而抑制肿瘤的血管生成。阿司匹林和舒林酸可通过抑制COX-2 及VEGF 的表达而抑制肿瘤的生长。

关键词: 阿司匹林, 舒林酸, COX-2, VEGF, CD₄₄V₆, LN-R

Abstract: AIM: To investigate the effects of aspirin and sulindac on the expression of cyclooxygenase-2(COX-2), vascular endothelial growth factor (VEGF), CD₄₄V₆ and laminin receptor (LN-R) in 3AO cells. METHODS: The growth inhibition effects of aspirin and sulindac in 3AO cells were tested by MTT assay.The expression of COX-2, VEGF, LN-R and CD₄₄V₆were evaluated by immunocytochemistry.RESULTS: Aspirin and sulindac significantly inhibited the growth of 3AO cells whether added before or after cells adhered.When the pH was adjusted with sodium bicarbonate, the inhibition had a little decrease.Aspirin and sulindac also inhibited the expression of COX-2 and VEGF.But there were no effect on the expression of LN-R and CD₄₄V₆.CONCLUSION: Inhibited expression of COX-2 may down-regulate the expression of VEGF, and inhibit the angiogenesis of tumor.Aspirin and sulindac may exert their chemopreventive and anticancer effects by inhibiting the expressions of COX-2 and VEGF.

Key words: aspirin, sulindac, cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, CD₄₄V₆

中图分类号:

R971.1

高雪芹, 韩金祥, 张维东, 宋守芹, 朱波. 阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD₄₄V₆表达的影响[J]. 中国临床药理学与治疗学, 2004, 9(8): 906-910.

GAO Xue-Qin, HAN Jin-Xiang, ZHANG Wei-Dong, SONG Shou-Qin, ZHU Bo. Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD₄₄V₆ in 3AO cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(8): 906-910.

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阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD₄₄V₆表达的影响

Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD₄₄V₆ in 3AO cells

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