纳洛酮对反复脑缺血再灌注小鼠学习记忆的影响及机制

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (8): 935-938.

纳洛酮对反复脑缺血再灌注小鼠学习记忆的影响及机制

霍展样, 霍小趁¹, 符凤英²

新乡医学院机能学实验室, 新乡453003, 河南;
¹博爱县医院肿瘤科, 博爱, 454400, 河南;
²北京大学医学部生理系, 北京100086

收稿日期:2004-04-21 修回日期:2004-06-17 出版日期:2004-08-26 发布日期:2020-11-20
通讯作者: 霍展样,女,学士,研究方向为脑缺血再灌注治疗与吗啡戒断治疗。Tel:0373-3029911 E-mail:huo_zhan_yang@sina.com

Effects and mechanism of naloxone on learning and memory in mice being repeatedly cerebral ischemia reperfusion

HUO Zhan-Yang, HUO Xiao-Chen¹, FU Feng-Ying²

Laboratory of Bio-Mechanism, Xinxiang Medical College, Xinxiang 453003, Hennan, China;
¹Department of tumor,Boai Hospital, Boai 454450, Hennan, China;
²Department of Physiology, Medical Department of Beijing University,Beijing 100086, China

Received:2004-04-21 Revised:2004-06-17 Online:2004-08-26 Published:2020-11-20

摘要/Abstract

摘要： 目的: 观察侧脑室注射(intra-cerebroventricular injection,i.c.v)纳洛酮(naloxone hydrochloride,NAL)对反复脑缺血再灌注小鼠学习记忆的影响及机制。方法: 小鼠作侧脑室埋植套管,术后抗感染4 ~ 5 d,然后采用清醒小鼠造成反复脑缺血再灌注模型,电迷宫法观察小鼠学习记忆情况,比色法检测脑内丙二醛含量,放射免疫法测定脑内及血浆内β-内啡呔(β-endorphin,β-Ep)含量。结果: 纳洛酮可以明显改善反复脑缺血再灌注小鼠学习记忆的情况,同时可不同程度地抑制由反复脑缺血再灌注引起的脑内异常升高的丙二醛、β-内啡呔,改善海马CA1 区细胞数目。结论: 纳洛酮对反复脑缺血再灌注损伤有保护作用,其机制可能与抑制脑内升高的丙二醛、β-内啡呔,从而提高海马CA1 区细胞数目有关。

关键词: 侧脑室注射, 反复脑缺血再灌, 学习记忆, 纳洛酮, 丙二醛, β-内啡呔;

Abstract: AIM: To observe the effects and mechanism of naloxone being injected intra-cere broventricular on studying and memory in mice with repeatedly cerebral ischemia reperfusion.METHODS: Double-tube was embedded in lateral ventricle of mice, anti-infection was taken for 4 or 5 days to mice after operation, and the repeated cerebral ischemia reperfusion model was made in awakening mice.Behavior of learning and memory was observed by eletic maze test, methylenedioxy (MDA) in brain tissue was detected by TBA, the content of β-Ep in brain tissue and blood plasma was measured by radio-immunity method, and the number of cell was counted in htppcoampal CA1 on section of brain tissue.RESULTS: Naloxone obviously improved the disorder of studying and memory of mice, and inhibited the abnormal increased MDA and β-Ep in brain tissue caused by repeated cerebral ischemia.It also improved the number of cell in htppcoampal CA1.CONCLUTION: Naloxone can protect mice being repeatedly cerebral ischemia reperfusion from the damage of studying and memory, and its mechanism is related to its contradicting β-Ep activity and lowing MDA in brain and increased htppcoampal CA1.

Key words: intra-cerebroventricular injection, repeated cerebral ischemia-reperfusion, naloxone hydrochloride, studying and memory, malonydialdehyde (MDA), β-endorphin ;, htppcoampal CA1

中图分类号:

R965.1

霍展样, 霍小趁, 符凤英. 纳洛酮对反复脑缺血再灌注小鼠学习记忆的影响及机制[J]. 中国临床药理学与治疗学, 2004, 9(8): 935-938.

HUO Zhan-Yang, HUO Xiao-Chen, FU Feng-Ying. Effects and mechanism of naloxone on learning and memory in mice being repeatedly cerebral ischemia reperfusion[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(8): 935-938.

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