染料木黄酮对哮喘患者核因子-κB 表达和肿瘤坏死因子-α分泌的影响

摘要/Abstract

摘要： 目的: 探讨染料木黄酮对支气管哮喘患者外周血单个核细胞(PBMCs) 核因子-κB(NF-κB) 表达及肿瘤坏死因子-α(TNF-α) 分泌的作用。方法: 32 例支气管哮喘急性发作期患者及31 例健康对照者的PBMCs, 均分空白对照、地塞米松和染料木黄酮3 组进行研究。NF-κB 表达由免疫组化染色检测, TNF-α含量由放射免疫法测定。结果: 哮喘患者PBMCsNF-κB 胞核染色阳性率及培养上清TNF-α含量明显高于健康对照者(P<0. 01), 且二者呈显著正相关(P<0. 01) 。染料木黄酮抑制哮喘组NF-κB 高表达及TNF-α高分泌, 与哮喘空白对照组比较二者均有统计学意义(P<0. 01), 但其抑制作用弱于地塞米松, 且哮喘染料木黄酮组PBMCs NF-κB 胞核染色阳性率与TNF-α含量呈显著正相关(P<0. 01) 。结论: 哮喘患者NF-κB 表达增高, TNF-α分泌增多。染料木黄酮可抑制哮喘患者PBMCs NF-κB 的高表达及TNF-α的高分泌, 对哮喘可能有潜在的治疗作用。

关键词: 染料木黄酮, 支气管哮喘, 核因子-κB, 肿瘤坏死因子-α

Abstract: AIM: To investigate the effects of genistein on the expression of nuclear factor-κB (NF-κB) and the levels of tumor necrosis factor-α(TNF-α) in peripheral blood mononuclear cells (PBMCs) of asthma patients.METHODS: PBMCs were isolated from blood samples of 32 asthma patients and 31 healthy persons, which was divided into control groups, dexamethasonetreated groups and genistein-treated groups. The expression of NF-κB was analyzed by immunocytochemical staining. The level of TNF-αwas measured by radioimmunoassay.RESULTS: The percentage of NF-κB-positive cells in PBMCs and the level of TNF-αin PBMCs culture supernatants were significantly higher in asthma patients than in healthy persons (all P<0. 01). There was a positive correlation between the percentage of NF-κB-positive cells and the level of TNF-αin asthma patients (P<0. 01). Genistein depressed the expression of NF-κB and the secretion of TNF-αof PBMCs in asthma patients. The percentage of NF-κB-positive cells in PBMCs and the level of TNF-αin PBMCs culture supernatants in asthmatic genistein-treated groups were both significantly lower than those of asthmatic control groups (all P<0. 01), but this depression was not so strong as dexamethasone. There was a positive correlation between the percentage of NF-κBpositive cells and the level of TNF-α in asthmatic genistein-treated groups (P<0. 01).CONCLUSION: There are increases in both expression of NF-κB and the level of TNF-α.Genistein can decrease the expression of NF-κB and the level of TNF-αin asthma patients, so it may has a hidalen effect in treating asthma.

Key words: genistein, asthma, nuclear factor-κB, tumor necrosis factor-α

中图分类号:

R977.1

古兴宇, 刘晓菊. 染料木黄酮对哮喘患者核因子-κB 表达和肿瘤坏死因子-α分泌的影响[J]. 中国临床药理学与治疗学, 2004, 9(9): 1011-1015.

GU Xing-Yu, LIU Xiao-Ju. Effects of genistein on expression of nuclear factor-κB and levels of tumor necrosis factor-αin asthma patients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(9): 1011-1015.

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