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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (8): 857-862.

• 研究原著 • 上一篇    下一篇

匹多莫德对免疫功能缺陷小鼠脾组织TNF-α和IL-6表达及相关免疫药理作用的研究

李慧, 王丙力, 毛一卿, 张翼, 李志红1, 申淑兰2, 谭焕然   

  1. 北京大学基础医学院药理学系, 北京 100083;
    1北京瑞康明药物研究所, 北京 100083;
    2海军总医院高干科, 北京 100037
  • 收稿日期:2005-03-21 修回日期:2005-04-29 发布日期:2020-11-22
  • 通讯作者: 谭焕然,女,教授,研究方向:分子药理学。Tel:010-82802004 E-mail:tanlab@sun.bjmu.edu.cn
  • 作者简介:李慧,女,讲师,研究方向:分子药理学。Tel:010-82802525 E-mail:lihuihaha@sina.com.cn

Effects and mechanisms of pidotimod on expression of TNF-αand IL-6 in immune-depressed mice spleen

LI Hui, WANG Bing-li, MAO Yi-qing, ZHANG Yi, LI Zhi-hong1, SHEN Shu-lan2, TAN Huan-ran   

  1. Department of Pharmacology, Health Science Center, Peking University, Beijing 100083, China;
    1Institute of Pharmacology, Beijing Ruikangming, Beijing 100083, China;
    2General Hospital of Navy, Beijing 100037, China
  • Received:2005-03-21 Revised:2005-04-29 Published:2020-11-22

摘要: 目的: 研究匹多莫德注射剂对正常小鼠及免疫功能低下小鼠脾组织TNF-α和IL-6表达及相关免疫功能的影响,以及匹多莫德免疫调节作用可能的机制。方法: RT-PCR法对小鼠脾组织的TNF-α和IL-6的表达进行分析;中性红法对小鼠的腹腔巨噬细胞的吞噬功能进行研究;MTT法检测ConA和LPS分别刺激的小鼠脾淋巴细胞的体外增殖;血清溶血素法检测小鼠血清抗体的产生。结果: 匹多莫德注射剂连续给药14d对正常小鼠及免疫功能低下小鼠脾组织的TNF-α和IL-6的表达有一定的促进作用;小鼠腹腔巨噬细胞的吞噬能力明显提高;ConA或LPS刺激的小鼠脾淋巴细胞的转化能力均有明显的提高。结论: 匹多莫德可明显提高正常小鼠和免疫功能低下小鼠的免疫功能,并促进TNF-α和IL-6表达。

关键词: 匹多莫德, 免疫活性, 巨噬细胞, 细胞因子, TNF-α, IL-6

Abstract: AIM: To study the influence of injection agent of pidotimod on function of the immune-system in normal and immune-depressed mice, and to investigate the mechanisms of the immunomodulating action of pidotimod.METHODS: The expressions of TNF-αand IL-6 in mice spleen was detected by RT-PCR method.The effects of pidotimod on phagocytosis functions of mice peritoneal exudate cells was investigated by neutral red phagocytosis.The lymphocyte proliferations induced by Con A or LPS were detected by MTT method.Adopt the serum haemolysis to measure the production of serum antibody.RESULTS: The normal and immune-depressed mice were treated for 14 days with different dosages of pidotimod by injection.Pidotimod can significantly increase the expressions of TNF-αand IL-6 of spleen, potentiate phagocytosis of the peritoneal exudate cells, potentiate the lymphocyte proliferations ability induced by Con A or LPS.CONCLUSION: Pidotimod can potentiate amelioration normal and immune-depressed mice immunesystem function and increase expressions of TNF-αand IL-6 of spleen.

Key words: pidotimod, immunocompetence, macrophages, cytokines, TNF-α, IL-6

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