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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (8): 867-870.

• 研究原著 • 上一篇    下一篇

过氧化氢对大鼠心室肌细胞L-型钙通道电流的影响

邓春玉, 林曙光, 钱卫民, 吴书林, 单志新, 杨敏, 余细勇   

  1. 广东省人民医院医学研究中心, 广东省心血管病研究所心内科, 广州 510080, 广东
  • 收稿日期:2005-05-30 修回日期:2005-07-11 发布日期:2020-11-22
  • 通讯作者: 邓春玉,男,博士,助理研究员;研究方向:心血管电生理。Tel:020-83827812-41203 E-mail:chunyudeng@tom.com
  • 作者简介:卫国,男,主管技师,主要从事感染研究工作。Tel:023-68765474 E-mail:weiguo@mail.tmmu.com.cn
  • 基金资助:
    广东省卫生厅基金项目(NoA2005046);广东省人民医院基金资助项目(NoY03041)

Effects of H2O2 on L-type calcium channel current in rat ventricular myocytes

DENG Chun-yu, LIN Shu-guang, QIAN Wei-min, WU Shu-lin, SHAN Zhi-xin, Yang Min, YU Xi-xiong   

  1. Division of Cardiology, Guangdong Provincial Cardiovascular Disease Institute, Research Center of Medical Science, Guangdong Provincial People's Hospital, Guangzhou 510080, Guangdong, China
  • Received:2005-05-30 Revised:2005-07-11 Published:2020-11-22

摘要: 目的: 研究过氧化氢(H2O2)对单个大鼠心室肌细胞L-型钙通道电流(Ica,L)的影响。方法: 用急性酶解法获得单个大鼠心室肌细胞;用标准的全细胞膜片钳技术记录钙通道电流,观察5mmol·L-1H2O2引起单个大鼠心室肌细胞Ica,L改变。结果: 在5mmol·L-1H2O2作用下,大鼠心室肌细胞Ica,L峰值电流密度从4.89±0.52pApF增加至9.80±0.65pApF(P<0.05,n=10),电流-电压曲线下移,但激活电位、峰电位和翻转电位无明显改变;H2O2对Ica,L激活时间常数-电压曲线无明显影响,但可使其灭活时间常数-电压曲线明显向上移;H2O2对Ica,L稳态激活曲线无明显改变,但可使其稳态失活曲线明显左移,V0.5从(-26.85±5.3)mV左移至(-37.20±4.5)mV(P<0.05,n=5)。结论: H2O2可以增加大鼠心肌细胞Ica,L,且使其失活明显减慢。

关键词: 过氧化氢, L-型钙通道电流, 心肌细胞, 全细胞膜片钳技术

Abstract: AIM: To determine the effect and mechanism of H2O2 on L-type calcium current (ICa, L) in single rat verntricular myocyte.METHODS: Whole-cell pacthclamp recording technique was used to record ICa, L in single cells isolated rat ventricular myocytes.RESULTS: The nifedipine-sensitive L-type calcium inward current was recorded in the rat ventricular myocytes.5 mmol·L-1 H2O2 increased the calcium channel current in a voltagedependent manner in ventricular myocytes of rats.The current-voltage curvewas shifted downwards, which active potential, peak potential and reverse potential had no significant changes.The time constants for inactivation (τ) are significantly greater (P <0.01) in the absence than the presence of 5 mmol·L-1 H2O2 over the entire range investigated, but its time constants for activation (τ) had no significant differences.The inactivation curve was shifted to more negative potential, but the activation curve was not altered.CONCLUSION: H2O2 can increase calcium channel current and slow channel inactivation in ventricular myocytes of rats.

Key words: H2O2, L-type calcium channel current, ventricular myocytes, whole-cell patch-clamp recording technique

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