人参皂苷Rb1预适应对肥厚乳鼠心肌细胞缺氧复氧损伤的保护作用

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (9): 1010-1014.

人参皂苷Rb1预适应对肥厚乳鼠心肌细胞缺氧复氧损伤的保护作用

郭佳, 刘小康, 武文, 张晓文¹

四川大学华西基础医学与法医学院药理教研室, 成都 610041, 四川;
¹广州中医药大学基础医学院中医基础教研室, 广州 510405, 广东

收稿日期:2005-01-20 修回日期:2005-04-26 发布日期:2020-11-22
通讯作者: 张晓文,男,博士,主要从事心血管药理研究。Tel:020-36588873 E-mail:Xiaowenzhang1@hotmail.com
作者简介:郭佳,女,硕士研究生,主要从事心血管药理研究。Tel:028-85501278 E-mail:guojiapharm@sina.com

Protective effects of preconditioning of Ginsenoside Rb1 on neonatal rat cardiomyocytes against hypoxia-reoxygenation injury

GUO Jia, LIU Xiao-kang, WU Wen, ZHANG Xiao-wen¹

Department of Pharmacology, West China School of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China;
¹Department of Basic Theory of Traditional Chinese Medicine, Basic Medicine College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong, China

Received:2005-01-20 Revised:2005-04-26 Published:2020-11-22

摘要/Abstract

摘要： 目的: 研究人参皂苷Rb1 对心肌细胞缺氧复氧(H/R) 损伤的保护作用。方法: 用血管紧张素II(Ang II) 刺激乳鼠心肌细胞肥厚, 以0.01 ~ 10μmol·L^-1 Rb1 对H/R 损伤心肌细胞预适应给药48h, 检测心肌细胞表面积(CSA)、细胞蛋白含量(TP)、细胞凋亡率、细胞生存率和乳酸脱氢酶(LDH)、丙二醛(MDA) 及一氧化氮(NO) 含量。结果: 与H/R 组相比, Rb1 组CSA 和TP 分别减少41.56%和43.37%;细胞生存率增加2.28 倍;凋亡率降低85.29%;LDH、MDA 含量分别降低59.20% 和72.03%;NO活性增加2.67 倍(P <0.01)。结论: Rb1 显著减轻肥厚心肌细胞H/R 损伤, 机制与抑制细胞凋亡、减少脂质过氧化和LDH 释放、增强NO 活性有关。

关键词: 人参皂苷Rb1, 心肌细胞肥厚, 缺氧复氧, 细胞凋亡, 丙二醛, 一氧化氮

Abstract: AIM: To study the effects and mechanisms of preconditioning of Ginsenoside Rb1 (Rb1) on neonatal rat cardiomyocytes against hypoxia-reoxygenation (H/R) injury.METHODS: Myocytes hypertrophy model was induced by Angiotensin II in neonatal rat and determined by assaying the cell surface area(CSA) and total protein content (TP) of cardiomyocytes.The H/R model was established after treatment with Rb1 for 48 h.The cell survival ability was assayed by MTT method.The apoptotic rate was assayed by flow cytometry.The activities of lactate dehydrogenase (LDH), content of nitric oxide (NO) and malondialdehyde (MDA) in medium were measured.RESULTS: After treatment of cells with Rb1 (0.01^-10.00 μmol·L^-1) for 48 h, compared with the H/R group, the myocyte CSA reduced by 41.56%, the TP lowered by 43.37%.The LDH activity reduced by 59.20%, the content of MDA decreased by 72.03%, the apoptosis rate reduced by 85.29%, the cell survival increased by 2.28 fold and NO activity enhanced by 2.67 fold (P <0.01).CONCLUSION: Ginsenoside Rb1 preconditioning can protect AngII-induced hypertrophy myocardium against H/R injury, increase myocyte viability and decrease total protein content.And these effects are related to reducing apoptosis and the release of LDH, improving activities of NO and decreasing the level of lipid peroxidation.

Key words: Ginsenoside Rb1, hypoxia-reoxygenation, hypertrophy, cardiomyocytes, LDH, apoptosis, nitric oxide, MDA

中图分类号:

R972

郭佳, 刘小康, 武文, 张晓文. 人参皂苷Rb1预适应对肥厚乳鼠心肌细胞缺氧复氧损伤的保护作用[J]. 中国临床药理学与治疗学, 2005, 10(9): 1010-1014.

GUO Jia, LIU Xiao-kang, WU Wen, ZHANG Xiao-wen. Protective effects of preconditioning of Ginsenoside Rb1 on neonatal rat cardiomyocytes against hypoxia-reoxygenation injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(9): 1010-1014.

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