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中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (10): 1102-1105.

• 研究原著 • 上一篇    下一篇

一种简单可靠的A549 裸小鼠原位接种模型的建立

康恺, 马秉亮1   

  1. 北京斯泰康生物技术有限公司, 北京 102206;
    1上海中医药大学中药学院药理学教研室, 上海 201203
  • 收稿日期:2006-05-30 接受日期:2006-06-14 出版日期:2006-10-26 发布日期:2020-11-05

A simple and repeatable orthotopic model of the A549 cell line in nude mice

KANG Kai, MA Bing-liang1   

  1. Beijing Starvax Biotechnology Incorporation, Life Park Road, Beijing 102206 , China;
    1Department of Pharmacy ,College of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203 , China
  • Received:2006-05-30 Accepted:2006-06-14 Online:2006-10-26 Published:2020-11-05
  • About author:KANG Kai,male, master of pharmacology, majoring in onco-pharmacology.Tel:010-80728608-208 ;13466369829 E-mail:bigoose 1979 @eyou.com

摘要: 目的 建立一种易操作、可靠的A549 原位接种模型。方法 人肺腺癌细胞株A549 悬浮于matrigel中, 接种于裸小鼠左肺或右侧前肢皮下, 观察肿瘤生长状况及化疗药卡铂的抗肿瘤作用。结果 肿瘤在胸腔中形成, 并扩展至对侧肺。与皮下肿瘤模型相比, 生长在肺中的肿瘤对卡铂不敏感。结论 该A549 原位接种模型简单可行, 可用于筛选新型抗肺癌活性化合物。

关键词: A549 细胞株, 原位接种模型, 裸小鼠

Abstract: AIM: To establish a simple and repeatable orthotopic animal model of the A549 cell line.METHODS: A549 cells in matrigel were inoculated into left lung or right flank of nude mouse.The growth pattern of A549 tumors and the chemotherapeutic efficacy of carboplatinum were studied.RESULTS: Tumors generated and dispersed widespreadly to contralateral lung.Tumors in lung were less susceptible to carboplatinum than those growing in flank.CONCLUSION: A simple and repeatable orthotopic animal model of the A549 cell line was presented,this allows researchers to evaluate novel compounds and new drugs for treatment of human lung cancer.

Key words: A549 cell line, orthotopic model, nude mice

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