Survivin 与bcl-2 基因反义寡核酸联合对人乳腺癌裸鼠皮下移植瘤的治疗作用

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (10): 1164-1167.

Survivin 与bcl-2 基因反义寡核酸联合对人乳腺癌裸鼠皮下移植瘤的治疗作用

王文武, 欧阳学农, 张霞, 姜浩¹

南京军区福州总医院肿瘤科, 福州 350025 , 福建;
¹南华大学附属第一医院肿瘤科, 衡阳 421001 , 湖南

收稿日期:2006-01-13 修回日期:2006-02-28 出版日期:2006-10-26 发布日期:2020-11-05
通讯作者: 欧阳学农,男, 副主任医师, 硕士学位, 硕导, 研究方向:肿瘤内科的临床研究。Tel:13809516120 E-mail:oyxn@public.fz.fj.cn
作者简介:王文武, 男, 硕士, 主治医师, 研究方向:肿瘤防治。Tel:13950289825 E-mail:Wangwwu2003@Yahoo.com.cn

Therapeutic effects of survivin and bcl-2 gene combined with antisense oligodeoxynucleotide on human breast cancer transplanted subcutaneously in nude mice

WANG Wen-wu, OU-YANG Xue-nong, ZHANG Xia, JIANG Hao¹

Nanjing Military Command Fuzhou General Hospital , Fuzhou 350025 , Fujian , China;
¹Department of Oncology , the First Hospital Affiliated to Nanhua Uniuersity , Hengyang 421000 , Hunan , China

Received:2006-01-13 Revised:2006-02-28 Online:2006-10-26 Published:2020-11-05

摘要/Abstract

摘要： 目的本研究旨在探讨survivin 与bcl-2 基因反义寡脱氧核苷酸联合对人乳腺癌裸鼠皮下移植瘤的治疗作用。方法在BALB/c 裸鼠上建立MCF-7乳腺癌细胞株模型, 然后随机分成阴性对照组和5个实验组[ 分别为脂质体survivin 正义脱氧寡核苷酸(SODN)组、脂质体bcl-2 SODN 组、脂质体survivin 反义寡脱氧核苷酸(ASODN)组、脂质体bcl-2 ASODN组、联合反义给药组] 。检测裸鼠体重及肿瘤体积变化, 计算抑瘤率及肿瘤缩小率。结果给药后, 联合反义给药组的肿瘤体积抑瘤率为78.4%, 肿瘤重量抑瘤率为75.3%, 肿瘤缩小率为41.6%。在给药后,各组裸鼠体重变化无明显统计学差异(P>0.05)。结论 survivin 与bcl-2 基因反义寡核酸联合在体内能明显抑制肿瘤生长。

关键词: 反义寡脱氧核酸, Survivin, bcl-2, 乳腺肿瘤, 移植瘤

Abstract: AIM: To investigate the effects of surviving and bcl-2 combined with ASODN on the treatment of breast cancer xenograft in nude mice.METHODS: The model of xenografts derived from MCF-7 breast cancer cell was established in BALB/c nude mice , then they were randomly divided into a negative control grouPand 5 experimental groups [intraperitoneal injection of liposomesurvivin-SODN , liposome-bcl-2-SODN , liposome-survivin-ASODN , liposome-bcl-2-ASODN , combined ASODN].The tumor growth inhibitory rate and the tumor volume decreased rate were calculated.RESULTS: Survivin and bcl-2 combined with ASODN exhibited potent tumor growth inhibition.The tumor volume inhibitory rate was 78.4%;the tumor weight inhibitory rate was 75.3%;the tumor volume decreased rate was 41.6%for combined grouPpost-experiment of the 6 groups , and there was no significant difference on nude mice weight post-experiment.CONCLUSION: Survivin and bcl-2 combined with ASODN showed potent tumor growth inhibition in vivo.

Key words: antisense oligodeoxynucleotide, surviving, bcl-2, breast cancer, xenograft

中图分类号:

R965.2
R966

王文武, 欧阳学农, 张霞, 姜浩. Survivin 与bcl-2 基因反义寡核酸联合对人乳腺癌裸鼠皮下移植瘤的治疗作用[J]. 中国临床药理学与治疗学, 2006, 11(10): 1164-1167.

WANG Wen-wu, OU-YANG Xue-nong, ZHANG Xia, JIANG Hao. Therapeutic effects of survivin and bcl-2 gene combined with antisense oligodeoxynucleotide on human breast cancer transplanted subcutaneously in nude mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(10): 1164-1167.

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