阿托伐他汀对金黄地鼠糖尿病模型巨噬细胞胆固醇外流的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (12): 1355-1359.

阿托伐他汀对金黄地鼠糖尿病模型巨噬细胞胆固醇外流的影响

朱颖, 严晓伟, 陈连凤, 王晋峰, 方全

中国医学科学院中国协和医科大学北京协和医院心内科, 北京 100730

收稿日期:2006-06-15 修回日期:2006-07-12 出版日期:2006-12-26 发布日期:2020-11-06
通讯作者: 严晓伟, 男, 医学博士, 教授, 博士生导师, 研究方向:心血管病学和脂质代谢异常。Tel:010-65295004 　E-mail:xswypumc@yahoo.com.cn
作者简介:朱颖, 女, 医学博士, 主治医生, 研究方向:内科学。

Effect of atorvastatin on intracellular cholesterol content and efflux of macrophages separated from diabetic hamster

ZHU Ying, YAN Xiao-wei, CHEN Lian-feng, WANG Jing-feng, FANG Quan

Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and PUMC,Beijing 100730, China

Received:2006-06-15 Revised:2006-07-12 Online:2006-12-26 Published:2020-11-06

摘要/Abstract

摘要： 目的研究不同剂量阿托伐他汀对糖尿病动物模型体内巨噬细胞胆固醇含量的影响, 进一步探讨他汀类药物抑制动脉粥样硬化斑块进展的机制。方法建立糖尿病金黄地鼠动物模型, 体外培养腹腔巨噬细胞, 在体外与不同剂量阿托伐他汀孵育, 利用酶法结合高效液相色谱法测定细胞内胆固醇及胆固醇脂含量。RT-PCR 方法检测细胞ABCA1 mRNA 表达量的不同。结果在apoA₁存在时, 阿托伐他汀可以显著促进巨噬细胞内胆固醇外流, 可能与药物增强细胞内胆固醇酯水解酶活性、促进巨噬细胞ABCA1的表达有关。结论阿托伐他汀促进ABCA1 介导的细胞内胆固醇外流。

关键词: 巨噬细胞, 动脉粥样硬化, 阿托伐他汀, 胆固醇外流

Abstract: AIM: To examine the effect of atorvastatin on the intercellular cholesterol content and efflux of diabetic golden hamster models, and to further explore the mechanism about inhibition of atorvastatin on progress of atherosclerotic score.METHODS: We established diabetic golden hamstermodels.Peritoneal macrophages were isolated, purified and cultured in vitro.Then macrophages were incubated with atorvastatin of different concentrations in vitro.Intracellular cholesterol content was determined with combination of enzymatic method and HPLC before and after atorvastatin treatments.RT-PCR was carried out to examine the expression of ABCA1mRNA in macrophages.RESULTS: Atorvastatin, by cooperation with apoA1, can promote intracellular cholesterol efflux significantly, which may be mediated by activation of cholesterol ester hydrolase and by promotion of ABCA1 expression in macrophages.CONCLUSION: Atorvastatin can increase ABCA1 mediated intracellular cholesterol efflux.

Key words: macrophage, atherosclerosis, atorvastatin, cholesterol efflux

中图分类号:

R965.2

朱颖, 严晓伟, 陈连凤, 王晋峰, 方全. 阿托伐他汀对金黄地鼠糖尿病模型巨噬细胞胆固醇外流的影响[J]. 中国临床药理学与治疗学, 2006, 11(12): 1355-1359.

ZHU Ying, YAN Xiao-wei, CHEN Lian-feng, WANG Jing-feng, FANG Quan. Effect of atorvastatin on intracellular cholesterol content and efflux of macrophages separated from diabetic hamster[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(12): 1355-1359.

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