白藜芦醇对离体犬冠状动脉血管环的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (3): 319-323.

白藜芦醇对离体犬冠状动脉血管环的影响

陈亮, 唐建华¹, 黄煌

湘南学院基础医学部,郴州 423000,湖南;
¹中南大学基础医学院,长沙 410000,湖南

收稿日期:2006-02-20 修回日期:2006-03-03 出版日期:2006-03-26 发布日期:2020-12-04
通讯作者: 唐建华,男,硕士生导师,教授,主要从事分子药理与生理的研究工作。Tel:0731-4495990 E-mail:miket561@xysm.net
作者简介:陈亮,男,研究生在读,副教授,主要从事心脑血管病的基础研究工作。Tel:13327358513 E-mail:chenliang8122@163.com

Effects of resveratrol on isolated coronary artery rings of canine

CHEN Liang, TANG Jian-Hua¹, HUANG Huang

Department of Basic Medicine,Xiangnan University,Chenzhou 423000,Hunan,China;
¹College of Basic Medicine,Central South University,Changsha 410000,Hunan,China

Received:2006-02-20 Revised:2006-03-03 Online:2006-03-26 Published:2020-12-04

摘要/Abstract

摘要： 目的研究白藜芦醇和17β-雌二醇对狗冠状动脉舒张特征的影响及其机制。方法制备狗冠状动脉血管环,固定于盛有K-H液的恒温肌槽内,并观察等长时血管张力的变化。结果白藜芦醇和17β-雌二醇使KCl及无钙K-H液中CaCl₂量效曲线明显右移,使肌条敏感性和最大收缩反应明显降低。Nω-L-硝基精氨酸、甲烯兰、正矾酸钠及去细胞内皮,可部分阻断白藜芦醇和17β-雌二醇的舒张血管作用。格列本脲组对白藜芦醇和17β-雌二醇的舒张作用没有影响。结论白藜芦醇和17β-雌二醇舒张血管都具有内皮依赖性,与K_ATP通道无关。

关键词: 白藜芦醇, 17β-雌二醇;, 冠状血管, 血管张力, 内皮细胞, 电压依赖性钙通道

Abstract: AIM: To investigate the relaxative characteristicsof resveratrol (RES) and 17β-Estradiol (EST)on coronary arteries in dogs and its mechanism.METHODS: Rings of canine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution,and then isometric tension was measured.RESULTS: Both RES and EST caused the does-response contractile curve of KCl in K-H solution and CaCl₂ in Ca²⁺-free K-H solution shift rightward,and their sensitivies and the values of maximum contractions had been decreased.In the Nω-Lnitro-arginine,Methylene Blue and Sodium Orthovanadate groups,the vasorelaxant effects of both estrogens were markedly attenuated (P<0.01),but there was no change in the grouPof Glibenclamide (P>0.05).CONCLUSION: Resveratrol and 17β-Estradiol relaxe vascular smooth muscle in an endothelium-dependent manner,and are not related to KATPchannel.

Key words: resveratrol, 17β-estradiol, coronary artery, vascular tension, endothelium, voltage-dependent calcium channel

中图分类号:

R331.32

陈亮, 唐建华, 黄煌. 白藜芦醇对离体犬冠状动脉血管环的影响[J]. 中国临床药理学与治疗学, 2006, 11(3): 319-323.

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