抗人TLR9 抗体对CpG 寡核苷酸介导的人外周血单核细胞肿瘤坏死因子-α释放的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (3): 324-327.

抗人TLR9 抗体对CpG 寡核苷酸介导的人外周血单核细胞肿瘤坏死因子-α释放的影响

吴羽中, 丁国富, 周红

第三军医大学药理学教研室,重庆 400038

收稿日期:2006-02-09 修回日期:2006-03-07 出版日期:2006-03-26 发布日期:2020-12-04
通讯作者: 周红,女,教授,主要从事抗炎免疫药理学。Tel:023-68752266 E-mail:zhouh64@mail.tmmu.com.cn
作者简介:吴羽中,男,硕士,助教,主要从事:抗炎免疫药理学。Tel:023-68752265 E-mail:wuchong@mail.tmmu.com.cn

Effect of anti-huamn TLR9 antibody on CpG tumor necrosis factor induced tumor necrosis factor-αrelease from human peripheral blood mononuclear cells

WU Chong, DING Guo-fu, ZHOU Hong

Departmen of Pharmacology,Third Military Medical University,Chongqing 400038,China

Received:2006-02-09 Revised:2006-03-07 Online:2006-03-26 Published:2020-12-04

摘要/Abstract

摘要： 目的观察细胞膜上的TLR9在CpG寡核苷酸(ODN)激活的信号转导通路中的作用。方法采用抗TLR9抗体阻断人外周血单个核细胞(hPBMCs)表面的TLR9,观察细胞膜表面的TLR9是否与CpGODN的内化和肿瘤坏死因子-α(TNF-α)的释放直接相关。结果虽然CpGODN和GpCODN都能被细胞吞噬,但只有CpGODN能够引起TNF-α释放;氯喹作为内体成熟抑制剂,在抑制这两种寡核苷酸降解的过程中无显著性差别;当使用抗人TLR9抗体阻断细胞膜表面的TLR9,对CpGODN的内化、CpGODN诱导的TNF-α释放以及氯喹对CpGODN诱导hPBMCs释放TNF-α的抑制作用均没有显著影响。结论细胞膜上的TLR9对CpGODN的内化和hPBMCs释放TNF-α没有直接影响。

关键词: CpG寡核苷酸, TLR9, 氯喹, 肿瘤坏死因子-α, 内化

Abstract: AIM: To observe the role of cell-surface TLR9 during TLR9-mediated signal transduction pathways.METHODS: Using anti-huamn TLR9 antibody to blockade cell-surface TLR9,internalization of CpG oligodeoxynucleotide(ODN) and tumor necrosis factor-α(TNF-α) release in human peripheral blood mononuclear cells (hPBMCs) were investigated.RESULTS: Both of CpG ODN and GpC ODN were endocytosed by the cells,only CpG ODN could induce TNF-αrelease.Addition of chloroquine (CQ),an inhibitor of endosome mature,delayed degradation of both ODN molecules,with no obvious difference between them.Importantly,blockade of cell-surface TLR9 using an anti-hTLR9 antibody did not significantly affect CpG ODN internalization,CpG ODNinduced TNF-αrelease,or CQ-induced inhibition of TNF-αrelease in hPBMCs.CONCLUSION: Cell-surface TLR9 does not directly participate in internalization of CpG ODN or TNF-αrelease from hPBMCs.

Key words: CpG ODN, TLR9, TNF-α, internalization, chloroquine

中图分类号:

吴羽中, 丁国富, 周红. 抗人TLR9 抗体对CpG 寡核苷酸介导的人外周血单核细胞肿瘤坏死因子-α释放的影响[J]. 中国临床药理学与治疗学, 2006, 11(3): 324-327.

参考文献

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