非诺贝特对溶血卵磷脂诱导的血管内皮细胞增生及凋亡的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (5): 535-539.

非诺贝特对溶血卵磷脂诱导的血管内皮细胞增生及凋亡的影响

孙国举, 谢秀梅, 邢莹¹, 杨天伦, 余国龙

中南大学湘雅医院心内科, 长沙 410008, 湖南;
¹郑州大学医学院干细胞研究中心, 郑州 450052, 河南

收稿日期:2006-02-05 修回日期:2006-04-20 出版日期:2006-05-26 发布日期:2020-12-09
通讯作者: 谢秀梅, 女, 教授, 博士生导师, 主要从事心血管内科疾病的研究。 Tel:13873181882 　E-mail: heart815@126.com
作者简介:孙国举, 男, 在读博士, 主任医师, 研究方向:冠心病。 Tel:13574156495 　 E-mail:sun8150@126.com

Effects of fenofibrate on proliferation and apoptosis induced by lysophos-phatidylcholine in cultured human umbilical vein endothelial cells

SUN Guo-ju, XIE Xiu-mei, XING Ying¹, YANG Tian-lun, YU Guo-long

Department of Cardiology, Xiang-Ya Hospital, Central SouthUniversity, Changsha 410008, Hunan, China;
¹the Stem Cell Research Center, Medical College, Zhengzhou University, Zhengzhou 450052, Henan, China

Received:2006-02-05 Revised:2006-04-20 Online:2006-05-26 Published:2020-12-09

摘要/Abstract

摘要： 目的: 观察非诺贝特对溶血卵磷脂(LPC) 诱导的血管内皮细胞增生、凋亡的影响并探讨其机制。 方法: 体外培养人脐静脉内皮细胞(HUVECs), 分为正常对照组、 LPC 组、非诺贝特低浓度组 (10 μmol·L^-1) 、非诺贝特中浓度组(50 μmol·L^-1) 及非诺贝特高浓度组(100 μmol·L^-1)。分别观测 LPC 对血管内皮细胞增生、凋亡及凋亡调控蛋白抑制 X 连锁凋亡抑制蛋白(XIAP) 的影响, 及非诺贝特干预后的变化。结果: 与正常对照组比较, LPC 抑制内皮细胞增生, 促进内皮细胞凋亡, XIAP 表达减弱。非诺贝特可干预 LPC 对内皮细胞的作用, 使内皮细胞增生增强, 凋亡减少, XIAP 表达增强。 结论: 非诺贝特可通过促进 XIAP 表达干预 LPC 对 HUVECs 增生及凋亡的影响。

关键词: 非诺贝特, 人脐静脉内皮细胞, 增生, 凋亡, X连锁凋亡抑制蛋白

Abstract: AIM: To investigate the effect of fenofi-brate on proliferation and apoptosis induced by lysophos-phatidylcholine(LPC) in cultured human umbilical vein endothelial cells (HUVECs) and itsmechanism.METHODS: HUVECs were cultured in vitro.The study was designated to 5 groups:normal control;group LPC;low-concentration fenofibrate(10 μmol·L^-1) group;middle-concentration fenofibrate (50μmol·L^-1) group;high-con-centration fenofibrate(100 μmol·L^-1) group.Proliferation and apoptosis of HUVECs were evaluated by MTT assay, flow cytometry (FCM) and fluorescence microscopy.The expression of XIAP proteinwas examined by immunohisto-chemistry method.RESULTS: Compared with control group, LPC could inhibit the growth and induce apoptosis of HUVECs, and decrease the expression of XIAP protein in HUVECs.Fenofibrate could increase the growth and decrease the apoptosis of HUVECs, and enhance the ex-pression of XIAP protein in HUVECs.CONCLUSION: Fenofibrate can improve proliferation and apoptosis in-duced by lysophosphatidylcholine in HUVECs by enhanc-ing expression of XIAP protein.

Key words: fenofibrate, human umbilical vein endo-thelial cells, proliferation, apoptosis, XIAP

中图分类号:

孙国举, 谢秀梅, 邢莹, 杨天伦, 余国龙. 非诺贝特对溶血卵磷脂诱导的血管内皮细胞增生及凋亡的影响[J]. 中国临床药理学与治疗学, 2006, 11(5): 535-539.

SUN Guo-ju, XIE Xiu-mei, XING Ying, YANG Tian-lun, YU Guo-long. Effects of fenofibrate on proliferation and apoptosis induced by lysophos-phatidylcholine in cultured human umbilical vein endothelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(5): 535-539.

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