异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (5): 562-564.

异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响

王士雷, 王世端, 李瑜, 刘传圣, 潘维敏, 张林

青岛大学医学院附属医院麻醉科, 青岛 266003, 山东

收稿日期:2006-02-25 修回日期:2006-03-24 出版日期:2006-05-26 发布日期:2020-12-09
通讯作者: 王士雷, 男, 博士, 副主任医师, 硕士生导师, 研究方向:脏器功能保护。 Tel:0532-82911519 　 Tel:wshlei @yahoo.com.cn
基金资助:
青岛市科委基金项目(NoKZ-13)

Effects of propofol and isoflurane on general brain ischemia-reperfusion injury in rats

WANG Shi-lei, WANG Shi-duan, LI Yu, LIU Chuang-sheng, PAN Wei-ming, ZHANG Lin

Department of Anesthesiology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shan-dong, China

Received:2006-02-25 Revised:2006-03-24 Online:2006-05-26 Published:2020-12-09

摘要/Abstract

摘要： 目的: 观察异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响。方法: 雄性 SD 大鼠随机分为 2 组, 每组14 只, 分别在异丙酚和异氟醚麻醉下行全脑缺血 15 min, 每组各 7 只于再灌注后 30 min 和 3 d 测SOD活性和 MDA 含量或海马 CA1 区存活神经元计数。结果: 异丙酚组大鼠海马和皮层 SOD 活性显著高于异氟醚组(P<0.01), MDA 含量显著低于异氟醚组(P<0.01)。异丙酚组海马 CA1 区成活神经元计数显著多于异氟醚组(P<0.05)。 结论: 异丙酚对大鼠脑缺血再灌注损伤的保护作用强于异氟醚, 其机制与其减轻氧自由基损伤有关。

关键词: 二异丙酚, 异氟醚, 缺血再灌注, 海马

Abstract: AIM: To explore the effects of propofol and isoflurane on total cerebral ischemia-reperfusion inju-ry of rats.METHODS: Male SD rats were randomly di-vided into two grougs (n =14), which were respectively anesthetized with propofol and isoflurane under mechani-cal ventilation.Global cerebral ischemia was induced by occlusions of bilateral common carotid and vertebral arter-ies (four vessels).Each group was divided into two sub-groups according to reperfusion time:30 min for detecting MDA and SOD in brain tissues and 3 d for detecting sur-vived CA1 Cells of hippocampus.RESULTS: The cont-ents of MDA was more after 30 min reperfusion in isoflu-rane group than that in propofol group (P<0.01), whereas the activities of SOD was higher in propofol group than that in isoflurane group(P<0.01).There were more survived neurons in CA1 after 3 day reperfusion in propofol group than in isoflurane group(P<0.05). CONCLUSION: Propofol has stronger protection effect in general brain ischemia-reperfusion injury, the mecha-nism is associated with the activity of oxide free radical.

Key words: propofol, isoflurane, cerebral ischemia-reperfusion, hippocampus

中图分类号:

R965.1

王士雷, 王世端, 李瑜, 刘传圣, 潘维敏, 张林. 异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响[J]. 中国临床药理学与治疗学, 2006, 11(5): 562-564.

WANG Shi-lei, WANG Shi-duan, LI Yu, LIU Chuang-sheng, PAN Wei-ming, ZHANG Lin. Effects of propofol and isoflurane on general brain ischemia-reperfusion injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(5): 562-564.

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